Riboflavin (Vitamin B2) Accumulation Modulates Neuronal Cellular Homeostasis in Typical Brain Development and Cerebral Palsy

IF 3.8 3区 医学 Q2 BIOCHEMISTRY & MOLECULAR BIOLOGY
Eulália Rebeca Silva-Araújo, Eduardo Padrón-Hernández, Ana Elisa Toscano, Osmar Henrique dos Santos Júnior, Joaci Pereira dos Santos Júnior, Henrique José Cavalcanti Bezerra Gouveia, Adriana Soares de Carvalho, Janaína Viana de Melo, Luiz Alberto Reis Mattos-Júnior, Raul Manhães-de-Castro
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Abstract

The developing brain requires high energy demands and metabolic efforts to regulate oxidative stress and myelination. Early insults cause mitochondrial dysfunction and compromise these pathways, potentially leading to cerebral palsy (CP), a severe and incurable neurological disorder that begins in childhood. Through a rodent preclinical study, we demonstrated that vitamin B2 (riboflavin), administered at a high dose (100 mg/kg), is accumulated in healthy (B2C) or paralytic (B2CP) brains and participates in neurodevelopment. Redox homeostasis was maintained in B2C through decreased malondialdehyde and carbonyls and increased glutathione-S-transferase activity. In B2CP rodents, there was a reduction in carbonyls and increased superoxide dismutase activity. Mitochondrial morphometric analysis suggests that riboflavin treatment increases biogenesis in controls and reduces mitochondrial deformation in CP. Ultrastructural analysis revealed increased myelin sheath thickness in B2C. Additionally, myelin figure formation and mitochondrial and axonal disintegration in CP were reduced by B2. Our evidence supports vitamin B2 accumulation as a beneficial mechanism to support energy homeostasis and mitochondrial demands that occur during typical neurodevelopment or in the face of CP.

核黄素(维生素B2)积累调节典型脑发育和脑瘫的神经元细胞稳态
发育中的大脑需要高能量需求和代谢努力来调节氧化应激和髓鞘形成。早期损伤会导致线粒体功能障碍,破坏这些通路,可能导致脑瘫(CP),这是一种始于儿童时期的严重且无法治愈的神经系统疾病。通过啮齿动物临床前研究,我们证明了高剂量(100 mg/kg)的维生素B2(核黄素)在健康(B2C)或瘫痪(B2CP)大脑中积累并参与神经发育。B2C通过降低丙二醛和羰基以及增加谷胱甘肽- s转移酶活性来维持氧化还原稳态。在B2CP啮齿动物中,羰基减少,超氧化物歧化酶活性增加。线粒体形态分析表明,核黄素治疗增加了对照组的生物发生,减少了CP的线粒体变形。超微结构分析显示,B2C的髓鞘厚度增加。此外,B2可减少CP的髓鞘图形成和线粒体和轴突解体。我们的证据支持维生素B2的积累是一个有益的机制,支持能量稳态和线粒体需求,发生在典型的神经发育或面对CP。
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来源期刊
Neurochemical Research
Neurochemical Research 医学-神经科学
CiteScore
7.70
自引率
2.30%
发文量
320
审稿时长
6 months
期刊介绍: Neurochemical Research is devoted to the rapid publication of studies that use neurochemical methodology in research on nervous system structure and function. The journal publishes original reports of experimental and clinical research results, perceptive reviews of significant problem areas in the neurosciences, brief comments of a methodological or interpretive nature, and research summaries conducted by leading scientists whose works are not readily available in English.
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