The peculiarities of biochemical and morphological changes in the heart of the rats under chronic hypodynamia in the development of adrenalin damage of heart.

Abstract

Objective: Aim: To evaluate the state of oxidation processes and morphological changes in the heart of rats with chronic hypodynamia during the development of epinephrine heart damage (EHD)..

Patients and methods: Materials and Methods: The study was performed on 144 white male Wistar rats. The animals were divided into two groups: 1 - control, 2 - hypodynamia. For EHD, rats were injected once intraperitoneally with a 0.18% solution of adrenaline hydrotartrate at the rate of 0.5 mg/kg of weight. Stress was induced in rats from 2.5 to 4.0 months of age. The animals were constantly kept in cages with limited living space for 1.5 months. The concentration of diene and triene conjugates (DC, TC), Schiff's bases (SB), TBA-active products (TBA-ap), oxidatively modified proteins (OMP), activity of superoxide dismutase (SOD) and catalase (CAT) were determined in the heart. A morphological study of preparations stained with Azan-trichrome was carried out. All studies were performed in control, 1, 3, 7, 14 and 28 days after adrenaline injection.

Results: Results: In the I series DC and TC increased after 1 day of EHD, fell to control values after 3 days, and then had wave-like character (highest - after 14 days), SB decreased, TBA-ap increase (maximal after 14 days). OMP370 increased after 1 and 3 days, after 14 days they were higher than in control, and after 28 days they decreased. OMP430 were greater than the control in all terms, except 28 days. The activity of antioxidant enzymes was lower than the control at all times. Hypodynamia caused an increase of lipid peroxidation and a decrease in OMP. Hypodynamia leads to increase of CAT, and decrase of SOD. All indicators of SOD and CAT exceeded hihger level of the antioxidant indicators of animals of the II group at EHD. Biochemical changes are consistent with morphological. After injection of epinephrine, severe vascular disorders, edema, endothelial cell damage, microcirculatory disorders, hemorrhages in the surrounding tissues, and sclerosing of the walls of arteries and venules were observed. Morphological studies established higher disturbances in the II group.

Conclusion: Conclusions: Hypodynamia in rats causes an increase of lipid peroxidation products and catalase activity in the heart, but a decrease in the content of oxidatively modified proteins and superoxide dismutase activity. Adrenaline injection causes activation of lipid peroxidation, especially secondary forms, and an less accumulation in the content of oxidatively modified proteins. During the development of epinephrine heart damage, the activity of antioxidants is significantly higher in animals with chronic hypodynamia. Biochemical changes are consistent with morphological changes, and indicate more damage to the myocardium in the process of development of epinephrine heart damage in animals of the I group.