Generation of humanized bone for disease modeling using porcine adipose tissue-derived extracellular matrix scaffolds and human dental pulp stem cells.

4区生物学 Q4 Biochemistry, Genetics and Molecular Biology

Methods in cell biology Pub Date : 2025-01-01 Epub Date: 2025-07-17 DOI:10.1016/bs.mcb.2025.06.003

Iratxe Madarieta, Jon Luzuriaga, Jone Salvador-Moya, Beatriz Pardo-Rodríguez, Patricia García-Gallastegui, Nerea García-Urkia, Igor Irastorza, Francisco-Javier Fernandez-San-Argimiro, Verónica Uribe-Etxebarria, Lucía Jiménez-Rojo, Fernando Unda, Beatriz Olalde, Jose Ramon Pineda, Gaskon Ibarretxe

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引用次数: 0

Abstract

Human Dental Pulp Stem Cells (hDPSCs) represent a remarkable cell source for tissue engineering and regenerative medicine, offering significant potential for use in personalized medicine and autologous therapies. Decellularized extracellular matrix (ECM)-derived biological scaffolds show excellent properties for supporting cell delivery and growth in both in vitro and in vivo applications. These scaffolds provide essential biochemical cues that regulate cellular functions and offer a more accurate representation of the in vivo environment. Porcine decellularized adipose tissue (pDAT) is a very abundant source of ECM, constituting an ideal material for biologic scaffold preparation. The integration of hDPSCs with pDAT-derived ECM enables the patient-specific generation of diverse humanized tissues and their application in personalized drug screening platforms. This chapter details a three dimensional (3D) culture methodology utilizing hDPSCs and pDAT-derived scaffolds to engineer humanized bone tissue.

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利用猪脂肪组织来源的细胞外基质支架和人牙髓干细胞制备用于疾病建模的人源化骨。

人牙髓干细胞（hDPSCs）是组织工程和再生医学的重要细胞来源，在个性化医疗和自体治疗中具有重要的应用潜力。脱细胞细胞外基质（ECM）衍生的生物支架在体外和体内应用中都具有支持细胞传递和生长的优异性能。这些支架提供了调节细胞功能的基本生化线索，并提供了更准确的体内环境表征。猪脱细胞脂肪组织（pDAT）是一种非常丰富的ECM来源，是制备生物支架的理想材料。hDPSCs与pdat衍生的ECM的整合使患者特异性的多种人源化组织的生成及其在个性化药物筛选平台中的应用成为可能。本章详细介绍了利用hdpsc和pdat衍生支架来设计人源化骨组织的三维（3D）培养方法。

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来源期刊

Methods in cell biology 生物-细胞生物学

CiteScore

3.10

自引率

0.00%

发文量

125

审稿时长

3 months

期刊介绍： For over fifty years, Methods in Cell Biology has helped researchers answer the question "What method should I use to study this cell biology problem?" Edited by leaders in the field, each thematic volume provides proven, state-of-art techniques, along with relevant historical background and theory, to aid researchers in efficient design and effective implementation of experimental methodologies. Over its many years of publication, Methods in Cell Biology has built up a deep library of biological methods to study model developmental organisms, organelles and cell systems, as well as comprehensive coverage of microscopy and other analytical approaches.