Multiple isolation of ST398/t011 MRSA from patients in the first half of 2025 in Imam Reza Hospital, Bojnurd, North Eastern Iran

IF 3.6 3区 医学 Q1 MICROBIOLOGY
Ebrahim Golmakani , Roya Sadidi , Seyed Ahmad Hashemi , Ali Haghbin , Amir Azimian
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引用次数: 0

Abstract

Introduction

Methicillin-resistant Staphylococcus aureus (MRSA) remains a global health threat, with livestock-associated (LA-)MRSA ST398/t011 emerging in regions with human-animal contact. This study reports the first detection of ST398/t011 in clinical isolates from Bojnurd, northeastern Iran, alongside the hospital-associated ST239/t037 clone.

Methods

From January to April 2025, 242 clinical samples from Imam Reza Hospital were screened for S. aureus. MRSA isolates underwent antimicrobial susceptibility testing (CLSI M100-2023) and molecular characterization (mecA, mecC, vanA, cfr, PVL, tsst, sec, hla; SCCmec, agr, spa typing, MLST).

Results

Of 32 positive cultures, 7 MRSA isolates were identified (ST398/t011 [n = 4]; ST239/t037 [n = 3]). All harbored mecA and exhibited resistance to β-lactams, clindamycin, and gentamicin but remained susceptible to vancomycin/linezolid. The PVL gene was detected in one ST398/t011 isolate. ST398/t011 carried SCCmec V and agr I, while ST239/t037 had SCCmec III. Isolates were recovered from wound (n = 3), blood (n = 2), eye (n = 1), and urine (n = 1) samples, with patients aged 6–48 years.

Discussion

This first report of LA-MRSA ST398/t011 in northeastern Iran highlights potential zoonotic transmission risks in this agricultural region. Co-detection of PVL in ST398/t011 and multidrug-resistant ST239/t037 underscores the need for enhanced surveillance. Limitations include the small sample size, but findings warrant further One Health investigations to assess reservoirs and transmission dynamics.
伊朗东北部Bojnurd伊玛目礼萨医院2025年上半年从患者身上多次分离到ST398/t011 MRSA
耐甲氧西林金黄色葡萄球菌(MRSA)仍然是全球健康威胁,牲畜相关(LA-)MRSA ST398/t011出现在人与动物接触的地区。本研究报告了首次在伊朗东北部Bojnurd的临床分离株中检测到ST398/t011,以及与医院相关的ST239/t037克隆。方法于2025年1 - 4月对伊玛目礼萨医院242份临床样本进行金黄色葡萄球菌筛查。对分离的MRSA进行了药敏试验(CLSI M100-2023)和分子鉴定(mecA、mecC、vanA、cfr、PVL、tsst、sec、hla; SCCmec、agr、spa分型、MLST)。结果32株阳性培养物中,鉴定出7株MRSA (ST398/t011 [n = 4];ST239/t037 [n = 3])。所有细菌都携带mecA,并对β-内酰胺类、克林霉素和庆大霉素耐药,但对万古霉素/利奈唑胺敏感。在1株ST398/t011分离株中检测到PVL基因。ST398/t011携带SCCmec V和agr I,而ST239/t037携带SCCmec III。分离株分别来自伤口(n = 3)、血液(n = 2)、眼睛(n = 1)和尿液(n = 1),患者年龄6 ~ 48岁。伊朗东北部首次报告的LA-MRSA ST398/t011突出了该农业地区潜在的人畜共患病传播风险。在ST398/t011和耐多药ST239/t037中共同检测PVL,强调了加强监测的必要性。局限性包括样本量小,但研究结果值得One Health进一步调查,以评估水库和传播动态。
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来源期刊
CiteScore
9.70
自引率
0.00%
发文量
18
审稿时长
45 days
期刊介绍: Pathogen genome sequencing projects have provided a wealth of data that need to be set in context to pathogenicity and the outcome of infections. In addition, the interplay between a pathogen and its host cell has become increasingly important to understand and interfere with diseases caused by microbial pathogens. IJMM meets these needs by focussing on genome and proteome analyses, studies dealing with the molecular mechanisms of pathogenicity and the evolution of pathogenic agents, the interactions between pathogens and host cells ("cellular microbiology"), and molecular epidemiology. To help the reader keeping up with the rapidly evolving new findings in the field of medical microbiology, IJMM publishes original articles, case studies and topical, state-of-the-art mini-reviews in a well balanced fashion. All articles are strictly peer-reviewed. Important topics are reinforced by 2 special issues per year dedicated to a particular theme. Finally, at irregular intervals, current opinions on recent or future developments in medical microbiology are presented in an editorial section.
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