Ebrahim Golmakani , Roya Sadidi , Seyed Ahmad Hashemi , Ali Haghbin , Amir Azimian
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引用次数: 0
Abstract
Introduction
Methicillin-resistant Staphylococcus aureus (MRSA) remains a global health threat, with livestock-associated (LA-)MRSA ST398/t011 emerging in regions with human-animal contact. This study reports the first detection of ST398/t011 in clinical isolates from Bojnurd, northeastern Iran, alongside the hospital-associated ST239/t037 clone.
Methods
From January to April 2025, 242 clinical samples from Imam Reza Hospital were screened for S. aureus. MRSA isolates underwent antimicrobial susceptibility testing (CLSI M100-2023) and molecular characterization (mecA, mecC, vanA, cfr, PVL, tsst, sec, hla; SCCmec, agr, spa typing, MLST).
Results
Of 32 positive cultures, 7 MRSA isolates were identified (ST398/t011 [n = 4]; ST239/t037 [n = 3]). All harbored mecA and exhibited resistance to β-lactams, clindamycin, and gentamicin but remained susceptible to vancomycin/linezolid. The PVL gene was detected in one ST398/t011 isolate. ST398/t011 carried SCCmec V and agr I, while ST239/t037 had SCCmec III. Isolates were recovered from wound (n = 3), blood (n = 2), eye (n = 1), and urine (n = 1) samples, with patients aged 6–48 years.
Discussion
This first report of LA-MRSA ST398/t011 in northeastern Iran highlights potential zoonotic transmission risks in this agricultural region. Co-detection of PVL in ST398/t011 and multidrug-resistant ST239/t037 underscores the need for enhanced surveillance. Limitations include the small sample size, but findings warrant further One Health investigations to assess reservoirs and transmission dynamics.
期刊介绍:
Pathogen genome sequencing projects have provided a wealth of data that need to be set in context to pathogenicity and the outcome of infections. In addition, the interplay between a pathogen and its host cell has become increasingly important to understand and interfere with diseases caused by microbial pathogens. IJMM meets these needs by focussing on genome and proteome analyses, studies dealing with the molecular mechanisms of pathogenicity and the evolution of pathogenic agents, the interactions between pathogens and host cells ("cellular microbiology"), and molecular epidemiology. To help the reader keeping up with the rapidly evolving new findings in the field of medical microbiology, IJMM publishes original articles, case studies and topical, state-of-the-art mini-reviews in a well balanced fashion. All articles are strictly peer-reviewed. Important topics are reinforced by 2 special issues per year dedicated to a particular theme. Finally, at irregular intervals, current opinions on recent or future developments in medical microbiology are presented in an editorial section.