Preparation, characterization, and antioxidant activity of bovine bone peptide-calcium chelate against oxidative stress-induced injury in Caco-2 cells

IF 4.7 2区医学 Q1 BIOCHEMISTRY & MOLECULAR BIOLOGY

Bioorganic Chemistry Pub Date : 2025-09-05 DOI:10.1016/j.bioorg.2025.108958

Aqing Jian , Baide Mu , Kun Li , Qingyi Deng , Juan Wang , Chunxiang Piao , Tingyu Li , Mingxun Cui , Guanhao Li , Hongmei Li

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Abstract

In this study, bovine peptide‑calcium chelates (BBP-Ca) were prepared via enzymatic hydrolysis to generate peptides and fermentation to obtain soluble calcium ions, which were then chelated together. The structural characteristics of BBP-Ca were comprehensively analyzed using FTIR, SEM, and UV spectroscopy. Additionally, its antioxidant capacity was evaluated by examining its protective effects against oxidative stress-induced damage in Caco-2 cells. The effect of BBP-Ca on the H₂O₂-induced model demonstrated its ability to inhibit ROS generation, reduce MDA levels, and enhance the activities of SOD, CAT, and GSH-Px. Furthermore, BBP-Ca was found to upregulate the mRNA expression of the nuclear factor erythroid-2-related factor 2 (Nrf2), which in turn increased the expression of the antioxidant enzymes heme oxygenase-1 (HO − 1) and NAD(P)H quinone oxidoreductase 1 (NQO1), while also downregulating the expression of Kelch-like ECH-associated protein 1 (Keap1). These findings suggest that BBP-Ca can mitigate H₂O₂-induced oxidative damage in Caco-2 cells via the Nrf2 signaling pathway. This study promoted the progress in the field of food-source mineral supplements by directly preparing peptide‑calcium chelates from bovine bones, avoiding synthetic additives.

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牛骨肽-钙螯合物的制备、表征及其抗氧化应激诱导Caco-2细胞损伤的活性研究

在本研究中，牛肽钙螯合物（BBP-Ca）通过酶解生成多肽和发酵获得可溶性钙离子，然后将它们螯合在一起。利用FTIR、SEM和UV光谱综合分析了BBP-Ca的结构特征。此外，通过检测其对Caco-2细胞氧化应激诱导损伤的保护作用来评估其抗氧化能力。BBP-Ca对h2o2诱导模型的影响表明其能够抑制ROS的产生，降低MDA水平，提高SOD、CAT和GSH-Px的活性。此外，BBP-Ca上调核因子红细胞2相关因子2 （Nrf2）的mRNA表达，从而增加抗氧化酶血红素加氧酶-1 （HO−1）和NAD(P)H醌氧化还原酶1 （NQO1）的表达，同时下调kelch样ech相关蛋白1 （Keap1）的表达。这些发现表明BBP-Ca可以通过Nrf2信号通路减轻h2o2诱导的Caco-2细胞氧化损伤。本研究通过直接从牛骨中制备肽钙螯合物，避免了人工合成添加剂，促进了食品源矿物质补充剂领域的进展。

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来源期刊

Bioorganic Chemistry 生物-生化与分子生物学

CiteScore

9.70

自引率

3.90%

发文量

679

审稿时长

31 days

期刊介绍： Bioorganic Chemistry publishes research that addresses biological questions at the molecular level, using organic chemistry and principles of physical organic chemistry. The scope of the journal covers a range of topics at the organic chemistry-biology interface, including: enzyme catalysis, biotransformation and enzyme inhibition; nucleic acids chemistry; medicinal chemistry; natural product chemistry, natural product synthesis and natural product biosynthesis; antimicrobial agents; lipid and peptide chemistry; biophysical chemistry; biological probes; bio-orthogonal chemistry and biomimetic chemistry. For manuscripts dealing with synthetic bioactive compounds, the Journal requires that the molecular target of the compounds described must be known, and must be demonstrated experimentally in the manuscript. For studies involving natural products, if the molecular target is unknown, some data beyond simple cell-based toxicity studies to provide insight into the mechanism of action is required. Studies supported by molecular docking are welcome, but must be supported by experimental data. The Journal does not consider manuscripts that are purely theoretical or computational in nature. The Journal publishes regular articles, short communications and reviews. Reviews are normally invited by Editors or Editorial Board members. Authors of unsolicited reviews should first contact an Editor or Editorial Board member to determine whether the proposed article is within the scope of the Journal.