Sustainable synthesis and functional profiling of Ipomoea hederifolia-derived terpenoids-assisted silver nanoparticles: Mechanistic insights into anticancer, antioxidant, antibiofilm, and anti-quorum sensing activities

IF 2.2 3区生物学 Q2 BIOCHEMISTRY & MOLECULAR BIOLOGY

Biophysical chemistry Pub Date : 2025-09-04 DOI:10.1016/j.bpc.2025.107524

Khushboo Makwana , Reem Binsuwaidan , Mohd Adnan , Nawaf Alshammari , Mitesh Patel

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Abstract

Silver nanoparticles (AgNPs) synthesized through green chemistry approaches offer a sustainable alternative to conventional methods, with potential applications in various biological fields. In this study, we report the synthesis of AgNPs using terpenoids derived from Ipomoea hederifolia L. (Convolvulaceae). The AgNPs (AgNPs-T) were characterized using UV–Vis spectroscopy, which revealed a surface plasmon resonance (SPR) peak at 452 nm, confirming successful synthesis. Fourier-transform infrared spectroscopy (FTIR) analysis identified functional groups such as hydroxyl and carbonyl that facilitated the reduction of silver ions and acted as stabilizing agents. Transmission electron microscopy (TEM) showed that the AgNPs-T were spherical in shape, with sizes ranging from 4 to 20 nm, and were well-dispersed due to the presence of capping agents from the plant extract. The biological activities of AgNPs-T were evaluated, showcasing potent antibacterial activity against several human pathogenic bacteria. Additionally, AgNPs-T exhibited significant antibiofilm and anti-quorum sensing activities, disrupting biofilm formation and inhibiting bacterial communication. The nanoparticles also demonstrated strong antioxidant properties by scavenging DPPH radicals in a dose-dependent manner. Moreover, cytotoxicity studies using the MTT assay revealed that AgNPs-T exerted dose-dependent anticancer effects against breast cancer (MCF-7) cells. These findings suggest that Ipomoea hederifolia-derived AgNPs possess multifunctional biological activities, making them promising candidates for applications in antimicrobial, antioxidant, and anticancer therapies.

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Ipomoea hederifolia衍生萜类辅助银纳米颗粒的可持续合成和功能分析：抗癌、抗氧化、抗生物膜和抗群体感应活性的机制见解

通过绿色化学方法合成银纳米粒子（AgNPs）是一种可持续的替代传统方法的方法，在各种生物领域具有潜在的应用前景。在本研究中，我们报道了利用从旋花科植物中提取的萜类化合物合成AgNPs的方法。利用紫外可见光谱对合成的AgNPs （AgNPs- t）进行了表征，发现在452nm处存在表面等离子体共振峰（SPR），证实了合成成功。傅里叶变换红外光谱（FTIR）分析发现，羟基和羰基等官能团有助于银离子的还原，并起到稳定剂的作用。透射电子显微镜（TEM）显示，AgNPs-T呈球形，大小在4 ~ 20 nm之间，由于植物提取物中的盖层剂的存在，AgNPs-T分散良好。对AgNPs-T的生物活性进行了评价，显示出对几种人类致病菌的强抑菌活性。此外，AgNPs-T表现出显著的抗生物膜和反群体感应活性，破坏生物膜的形成并抑制细菌的交流。纳米颗粒还表现出很强的抗氧化性能，清除DPPH自由基，并呈剂量依赖性。此外，使用MTT试验的细胞毒性研究表明，AgNPs-T对乳腺癌（MCF-7）细胞具有剂量依赖性的抗癌作用。这些发现表明，山茱萸衍生的AgNPs具有多种生物学活性，在抗菌、抗氧化和抗癌治疗方面具有广阔的应用前景。

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来源期刊

Biophysical chemistry 生物-生化与分子生物学

CiteScore

6.10

自引率

10.50%

发文量

121

审稿时长

20 days

期刊介绍： Biophysical Chemistry publishes original work and reviews in the areas of chemistry and physics directly impacting biological phenomena. Quantitative analysis of the properties of biological macromolecules, biologically active molecules, macromolecular assemblies and cell components in terms of kinetics, thermodynamics, spatio-temporal organization, NMR and X-ray structural biology, as well as single-molecule detection represent a major focus of the journal. Theoretical and computational treatments of biomacromolecular systems, macromolecular interactions, regulatory control and systems biology are also of interest to the journal.