SOX18 promotes lymphatic vessel regeneration to alleviate lymphedema by upregulating PROX1

IF 3.5 3区生物学 Q3 CELL BIOLOGY

Experimental cell research Pub Date : 2025-09-01 DOI:10.1016/j.yexcr.2025.114743

Xiang Zhou, Ping Li, Chunyan Li, Qiaoyan Dai, Jian Qi, Yi Yang

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引用次数: 0

Abstract

Promoting lymphatic vessel regeneration is an important method for repairing lymphedema. SOX18 can regulate lymphatic vessel development and plays a crucial role in promoting lymphatic vessel generation. This study aims to demonstrate the role of SOX18 in regulating lymphatic vessel regeneration for the repair of lymphedema and explore its related molecular mechanism. We used classical molecular biology methods such as RT-qPCR, Western blotting, CCK8 assay, Transwell assay, immunofluorescence, tube formation assay, dual luciferase assay, and CHIP assay to investigate the role of overexpressed SOX18 in promoting lymphatic vessel regeneration and its related molecular regulatory mechanisms. Subsequently, pathological histological analysis, Western blotting, and immunofluorescence were used to study the lymphedema mouse model. Overexpression of SOX18 promoted the proliferation, migration, and tube formation of LECs in vitro. Overexpression of SOX18 promoted lymphatic vessel regeneration for the repair of lymphedema in vivo. Mechanistically, SOX18 bound to the PROX1 promoter, promoted PROX1 transcription, activated the ERK pathway, upregulated VEGFR3, and promoted lymphatic vessel regeneration. This study demonstrates the important role of overexpressed SOX18 in regulating lymphatic vessel regeneration for the repair of lymphedema, which may provide new therapeutic options for lymphedema treatment.

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SOX18通过上调PROX1，促进淋巴管再生，减轻淋巴水肿。

促进淋巴管再生是修复淋巴水肿的重要方法。SOX18可以调节淋巴管发育，在促进淋巴管生成中起着至关重要的作用。本研究旨在证明SOX18在淋巴水肿修复中调节淋巴管再生的作用，并探讨其相关分子机制。我们采用RT-qPCR、Western blotting、CCK8、Transwell、免疫荧光、成管实验、双荧光素酶实验、CHIP等经典分子生物学方法，研究过表达SOX18在促进淋巴管再生中的作用及其相关分子调控机制。随后，采用病理组织学分析、Western blotting和免疫荧光法对淋巴水肿小鼠模型进行研究。SOX18的过表达促进了体外上皮细胞的增殖、迁移和成管。体内过表达SOX18可促进淋巴血管再生，修复淋巴水肿。机制上，SOX18结合PROX1启动子，促进PROX1转录，激活ERK通路，上调VEGFR3，促进淋巴管再生。本研究证实了过表达SOX18在调节淋巴血管再生修复淋巴水肿中的重要作用，可能为淋巴水肿治疗提供新的治疗选择。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Experimental cell research 医学-细胞生物学

CiteScore

7.20

自引率

0.00%

发文量

295

审稿时长

30 days

期刊介绍： Our scope includes but is not limited to areas such as: Chromosome biology; Chromatin and epigenetics; DNA repair; Gene regulation; Nuclear import-export; RNA processing; Non-coding RNAs; Organelle biology; The cytoskeleton; Intracellular trafficking; Cell-cell and cell-matrix interactions; Cell motility and migration; Cell proliferation; Cellular differentiation; Signal transduction; Programmed cell death.