Transcriptional Regulation of the Interferon Alpha-Inducible Protein-27 Like 2b by the Peroxisome Proliferator-Activated Receptor γ in Fatty Liver Disease

Abstract

Peroxisome proliferator-activated receptor γ (PPARγ) is a nuclear receptor abundantly expressed in the fatty liver of type 2 diabetic ob/ob mice. Herein, we investigated how PPARγ regulates the expression of the interferon alpha-inducible protein 27-like 2b (lfi27l2b) gene in the mouse liver. High expression of lfi27l2b was observed in the fatty liver of ob/ob mice, and the expression was further upregulated by PPARγ ligands; however, liver-specific Pparg knockout ameliorated this increase. Moreover, high lfi27l2b expression was observed in the fatty liver of alcohol-fed mice. Reporter assays indicated that the PPARγ-responsive element (PPRE) is necessary for PPARγ-dependent induction of Ifi27l2b promoter activities. Furthermore, electrophoretic mobility shift assays showed that PPARγ is capable of directly binding the PPRE. Overall, our results indicate that lfi27l2b is a novel PPARγ target in the fatty liver.