Endoplasmic reticulum stress modulation by Alpha-Pinene in a rotenone-induced rat model of Parkinson's disease

Abstract

Age is the most significant risk factor for Parkinson's disease, a common and progressive neurodegenerative disorder; however, exposure to toxic substances is also strongly implicated. Rotenone, an organic pesticide, induces neuropathological features of Parkinson's disease, and is widely used to create rodent models of the condition. Although the molecular mechanisms involved in the onset and progression of the disease are still unknown, neurodegenerative diseases due to protein accumulation in certain areas of the brain, have been associated with endoplasmic reticulum stress. Therefore, preventing or mitigating endoplasmic reticulum stress is considered a promising strategy for alleviating symptoms or slowing the progression of such diseases. In this study, the endoplasmic reticulum stress-related effect of Alpha Pinene, a herbal component, was investigated for the first time in a Rotenone-induced rat model of Parkinson's disease. 60 adult male rats were divided into 5 groups as Control, Vehicle, Rotenone (2 mg/kg), Alpha Pinene (50 mg/kg) and Alpha Pinene + Rotenone. After 28 days of experimental period, it was found that Alpha Pinen maintained the body weight of rats and prevented the deterioration of motor behaviours. Histological and immunohistochemical examinations of brain tissue revealed that it protected dopaminergic neurons and glial cells in the substantia nigra. Additionally, according to the biochemical analyses in the prefrontal cortex and mesencephalon regions of the brain, it was found that Alpha Pinene inhibited apoptosis and reduced endoplasmic reticulum stress. The results indicate that Alpha Pinene is a valuable compound for Parkinson's disease with its versatile neuroprotective effect.