MCS Ultrastructural Analyses Using Electron Microscopy.

Contact (Thousand Oaks (Ventura County, Calif.)) Pub Date : 2025-09-01 eCollection Date: 2025-01-01 DOI:10.1177/25152564251372668
Atsuki Nara
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引用次数: 0

Abstract

Membrane contact sites (MCSs) are microdomains that exchange ions and lipids between the membranes of two organelles. They facilitate the exchange of metabolites and act as a site for intracellular communication through material transport. Because of the important physiological significance of MCSs in localizing the exchange of substances and metabolic regulation, they are considered to play an important role in cell biology. Understanding MCS structure is essential for analyzing how substances move to and from each organelle. Several methods have been developed to analyze MCS function, with electron microscopy (EM) being the predominant technique when structural detail is needed. In this review, we summarize the ultrastructure of MCSs and how EM can be used to determine their role in cell biology.

Abstract Image

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电子显微镜下MCS超微结构分析。
膜接触位点(MCSs)是两个细胞器膜之间交换离子和脂质的微结构域。它们促进代谢物的交换,并通过物质运输作为细胞内通信的场所。由于MCSs在定位物质交换和代谢调节方面具有重要的生理意义,因此被认为在细胞生物学中发挥着重要作用。了解MCS结构对于分析物质如何进出每个细胞器至关重要。目前已经发展了几种分析MCS功能的方法,当需要结构细节时,电子显微镜(EM)是主要的技术。在这篇综述中,我们总结了MCSs的超微结构以及如何利用EM来确定它们在细胞生物学中的作用。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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CiteScore
3.10
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