Cell Function Experiments and Bioinformatics Analysis Jointly Revealed the Antineoplastic Effect of Lumican on Hepatocellular Carcinoma.

IF 6.2 Q2 GENETICS & HEREDITY

Phenomics (Cham, Switzerland) Pub Date : 2025-01-27 eCollection Date: 2025-06-01 DOI:10.1007/s43657-024-00182-w

Xiaoyu Zhou, Zixuan Xing, Ruijun Dong, Xi Zhang, Xuefeng Liang, Zhengyang Lu, Ganghua Yang

{"title":"Cell Function Experiments and Bioinformatics Analysis Jointly Revealed the Antineoplastic Effect of Lumican on Hepatocellular Carcinoma.","authors":"Xiaoyu Zhou, Zixuan Xing, Ruijun Dong, Xi Zhang, Xuefeng Liang, Zhengyang Lu, Ganghua Yang","doi":"10.1007/s43657-024-00182-w","DOIUrl":null,"url":null,"abstract":"Hepatocellular carcinoma (HCC) is one of the most common cancers worldwide, and immune checkpoint inhibitor (ICI)-based therapies are now an integral part of systemic treatment. In this study, we identify potential biomarker for HCC and further investigate its functional significance using both bioinformatic and experimental methods. Differential analysis and weighted gene co-expression network analysis (WGCNA) were conducted, identifying lumican (LUM) as the target gene. Our results showed a significant downregulation of LUM in HCC. LUM expression was also associated with the progression-free interval and the infiltration of B cells, neutrophils, and myeloid dendritic cells. Enrichment analysis highlighted the involvement of LUM in focal adhesion and the extracellular matrix. In addition, overexpression of LUM suppressed proliferation, migration and invasion in hepatoma cell lines while promoting cell apoptosis. Our results demonstrate the importance of LUM in HCC development and may help to elucidate the underlying mechanisms and biological processes influenced by LUM.Supplementary information: The online version contains supplementary material available at 10.1007/s43657-024-00182-w.","PeriodicalId":74435,"journal":{"name":"Phenomics (Cham, Switzerland)","volume":"5 3","pages":"252-269"},"PeriodicalIF":6.2000,"publicationDate":"2025-01-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12391580/pdf/","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Phenomics (Cham, Switzerland)","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.1007/s43657-024-00182-w","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"2025/6/1 0:00:00","PubModel":"eCollection","JCR":"Q2","JCRName":"GENETICS & HEREDITY","Score":null,"Total":0}

引用次数: 0

Abstract

Hepatocellular carcinoma (HCC) is one of the most common cancers worldwide, and immune checkpoint inhibitor (ICI)-based therapies are now an integral part of systemic treatment. In this study, we identify potential biomarker for HCC and further investigate its functional significance using both bioinformatic and experimental methods. Differential analysis and weighted gene co-expression network analysis (WGCNA) were conducted, identifying lumican (LUM) as the target gene. Our results showed a significant downregulation of LUM in HCC. LUM expression was also associated with the progression-free interval and the infiltration of B cells, neutrophils, and myeloid dendritic cells. Enrichment analysis highlighted the involvement of LUM in focal adhesion and the extracellular matrix. In addition, overexpression of LUM suppressed proliferation, migration and invasion in hepatoma cell lines while promoting cell apoptosis. Our results demonstrate the importance of LUM in HCC development and may help to elucidate the underlying mechanisms and biological processes influenced by LUM.

Supplementary information: The online version contains supplementary material available at 10.1007/s43657-024-00182-w.

查看原文本刊更多论文

细胞功能实验和生物信息学分析共同揭示了Lumican对肝细胞癌的抗肿瘤作用。

肝细胞癌（HCC）是世界范围内最常见的癌症之一，基于免疫检查点抑制剂（ICI）的治疗现在是全身治疗的一个组成部分。在本研究中，我们通过生物信息学和实验方法，确定潜在的HCC生物标志物，并进一步研究其功能意义。通过差异分析和加权基因共表达网络分析（WGCNA），确定lumican （LUM）为靶基因。我们的研究结果显示，在HCC中，LUM显著下调。LUM的表达也与无进展间隔和B细胞、中性粒细胞和骨髓树突状细胞的浸润有关。富集分析强调了LUM参与局灶黏附和细胞外基质。此外，过表达LUM可抑制肝癌细胞系的增殖、迁移和侵袭，促进细胞凋亡。我们的研究结果证明了LUM在HCC发展中的重要性，并可能有助于阐明受LUM影响的潜在机制和生物学过程。补充信息：在线版本包含补充资料，下载地址：10.1007/s43657-024-00182-w。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

求助全文

约1分钟内获得全文求助全文

来源期刊

Phenomics (Cham, Switzerland)

自引率

0.00%

发文量