Cytotoxic effects of flurbiprofen on rat adipose-derived stem cells.

IF 1.2 4区医学 Q4 PHARMACOLOGY & PHARMACY

Xenobiotica Pub Date : 2025-09-03 DOI:10.1080/00498254.2025.2552250

Jiamin Zeng, Xinyao Zhang, Lili Long, Beini Tan, Minghui Zhong, Fanwen Yang, Shicong Zheng, Zhe Yang, Fengyi Zhang, Lan Lan, Sida Liao

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引用次数: 0

Abstract

Adipose-derived stem cells (ADSCs) offer a novel approach for treating orthopaedic diseases. Flurbiprofen is a non-steroidal anti-inflammatory analgesic drug used in bone injury therapy. However, its cytotoxicity to ADSCs remains unclear.The differentiation potential of ADSCs was analysed in vitro using the differentiation induction method. Microscopic analysis, Live/Dead staining, MTT assay, and CCK8 assay were used to determine the cytotoxic effects of flurbiprofen on ADSCs at different concentration gradients. Vascular endothelial growth factor (VEGF) levels were measured by ELISA to reflect flurbiprofen's effects on the activity and paracrine function of ADSCs. ANOVA was applied, with p < 0.05 considered significant and p < 0.01 highly significant.Live cell concentration decreased dose-dependently with flurbiprofen treatment, particularly on the 7th day. The level of VEGF decreased significantly with the increase of the concentration of flurbiprofen in a certain concentration range, which indicated that flurbiprofen could inhibit the activity and secreted proteins of ADSCs.Our study showed that small doses of flurbiprofen did not affect the secretion of proteins by cells and cell activity. Therefore, it is necessary to pay attention to the concentration of flurbiprofen in the clinical application of ADSCs therapy for orthopaedic diseases.

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氟比洛芬对大鼠脂肪干细胞的细胞毒性作用。

1. 脂肪源性干细胞（ADSCs）为骨科疾病的治疗提供了新的途径。氟比洛芬是一种用于骨损伤治疗的非甾体抗炎镇痛药。然而，其对ADSCs的细胞毒性尚不清楚。采用诱导分化法分析ADSCs的体外分化潜能。采用显微分析、活/死染色、MTT法和CCK8法测定不同浓度梯度下氟比洛芬对ADSCs的细胞毒作用。ELISA法检测血管内皮生长因子（VEGF）水平，反映氟比洛芬对ADSCs活性及旁分泌功能的影响。2 .采用方差分析，P < 0.05认为显著，P < 0.01极显著。氟比洛芬治疗后，活细胞浓度呈剂量依赖性下降，特别是在第7天。在一定浓度范围内，随着氟比洛芬浓度的增加，VEGF水平明显下降，说明氟比洛芬可以抑制adscs的活性和分泌蛋白。我们的研究表明，小剂量氟比洛芬不影响细胞分泌蛋白质和细胞活性。因此，在临床应用ADSCs治疗骨科疾病时，有必要关注氟比洛芬的浓度。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Xenobiotica 医学-毒理学

CiteScore

3.80

自引率

5.60%

发文量

审稿时长

2 months

期刊介绍： Xenobiotica covers seven main areas, including:General Xenobiochemistry, including in vitro studies concerned with the metabolism, disposition and excretion of drugs, and other xenobiotics, as well as the structure, function and regulation of associated enzymesClinical Pharmacokinetics and Metabolism, covering the pharmacokinetics and absorption, distribution, metabolism and excretion of drugs and other xenobiotics in manAnimal Pharmacokinetics and Metabolism, covering the pharmacokinetics, and absorption, distribution, metabolism and excretion of drugs and other xenobiotics in animalsPharmacogenetics, defined as the identification and functional characterisation of polymorphic genes that encode xenobiotic metabolising enzymes and transporters that may result in altered enzymatic, cellular and clinical responses to xenobioticsMolecular Toxicology, concerning the mechanisms of toxicity and the study of toxicology of xenobiotics at the molecular levelXenobiotic Transporters, concerned with all aspects of the carrier proteins involved in the movement of xenobiotics into and out of cells, and their impact on pharmacokinetic behaviour in animals and manTopics in Xenobiochemistry, in the form of reviews and commentaries are primarily intended to be a critical analysis of the issue, wherein the author offers opinions on the relevance of data or of a particular experimental approach or methodology