Systematic Analysis of Lysine Succinylation in Streptococcus mutans.

IF 2.9 3区医学 Q1 DENTISTRY, ORAL SURGERY & MEDICINE

Molecular Oral Microbiology Pub Date : 2025-08-27 DOI:10.1111/omi.70007

Ting Tong, Ziyi Yang, Xiaoqiong Li, Qizhao Ma, Tao Gong, Dian Zhang, Jumei Zeng, Yuqing Li

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引用次数: 0

Abstract

Lysine succinylation (Ksuc) is a novel post-translational modification (PTM), which regulates biological functions in bacteria. Streptococcus mutans has been identified as a predominant cariogenic pathogen responsible for the initiation and progression of dental caries. However, lysine succinylation in S. mutans has not yet been investigated. In this study, a global lysine succinylome was analyzed to examine Ksuc in S. mutans. Overall, 2250 succinylated sites in 580 proteins were identified. Quantitative analysis demonstrated that Ksuc substrates were substantially altered in the biofilm growth state compared with the planktonic growth state. These differentially succinylated proteins were distributed across various cellular components and involved in crucial biological pathways, including translation, ribosomal structure, and biogenesis. Furthermore, lysine acetylation and succinylation extensively overlapped in S. mutans, and these bimodified proteins were associated with biofilm formation, glycolysis, and pyruvate metabolism. These results provided a foundation to further investigate the role of Ksuc in S. mutans pathogenicity and expand our understanding of Ksuc functions in bacterial physiology and virulence.

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变形链球菌赖氨酸琥珀酰化的系统分析。

赖氨酸琥珀酰化（Lysine succinylation, Ksuc）是一种新型的翻译后修饰（PTM），它调节着细菌的生物学功能。变形链球菌已被确定为一种主要的致龋病原体，负责龋齿的发生和发展。然而，在变形链球菌中赖氨酸琥珀酰化尚未被研究。在这项研究中，分析了一个全局赖氨酸琥珀酰酶来检测变形链球菌中的Ksuc。总共鉴定出580个蛋白中的2250个琥珀化位点。定量分析表明，与浮游生长状态相比，生物膜生长状态下k此类底物发生了实质性变化。这些不同琥珀酰化的蛋白分布在不同的细胞成分中，并参与关键的生物学途径，包括翻译、核糖体结构和生物发生。此外，赖氨酸乙酰化和琥珀酰化在变形链球菌中广泛重叠，这些双修饰蛋白与生物膜形成、糖酵解和丙酮酸代谢有关。这些结果为进一步研究Ksuc在S. mutans致病性中的作用奠定了基础，并扩大了我们对Ksuc在细菌生理和毒力中的作用的认识。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Molecular Oral Microbiology DENTISTRY, ORAL SURGERY & MEDICINE-MICROBIOLOGY

CiteScore

6.50

自引率

5.40%

发文量

审稿时长

>12 weeks

期刊介绍： Molecular Oral Microbiology publishes high quality research papers and reviews on fundamental or applied molecular studies of microorganisms of the oral cavity and respiratory tract, host-microbe interactions, cellular microbiology, molecular ecology, and immunological studies of oral and respiratory tract infections. Papers describing work in virology, or in immunology unrelated to microbial colonization or infection, will not be acceptable. Studies of the prevalence of organisms or of antimicrobials agents also are not within the scope of the journal. The journal does not publish Short Communications or Letters to the Editor. Molecular Oral Microbiology is published bimonthly.