Intermittent fasting and exercise: a dual intervention for orchestrating glycolipid conversion and utilization in healthy mice.

IF 3.7 2区 生物学 Q3 CELL BIOLOGY
Xueping Wu, Jian Shi, Junming Wang, Weiye Li, Jingxin Chen, Ruiying Lyu, Chunhong Liu
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Abstract

Intermittent fasting (IF) and exercise can reverse the impaired glycolipid conversion ability caused by obesity. However, the effects of IF and exercise combination on glycolipid conversion ability in normal individuals remain to be determined. In this study, male KM mice were subjected to IF (including alternate-day fasting (ADF), time-restricted fasting (TRF)), treadmill exercise, and a combination of the two interventions for 6 weeks. The effects of two IF models combined exercise on glycolipid conversion in mice were investigated by detecting serum biochemical indexes, and the expressions of genes and proteins closely related to the glycolipid conversion pathway in liver, gastrocnemius, and inguinal adipose tissue. The results showed that IF combined with exercise significantly reduced fasting blood glucose, glycated serum protein and liver glycogen levels in mice. TRF combined with exercise significantly decreased triglyceride levels in serum, liver and gastrocnemius. IF combined with exercise activated the ChREBP or SREBP1/FASN pathways to enhance the transcriptional activation of the glucose-mediated adipogenesis pathway, and simultaneously promoted the expression of fatty acid oxidation proteins and reduced liver gluconeogenesis genes expression, collectively improving lipid metabolic efficiency. Furthermore, IF increased the expression of glycogen turnover-related protein PPP1R3C and adipose thermogenesis-related protein UCP1 in the inguinal adipose tissue, indicating enhanced glycogen flux coordination with adipose thermogenic activation. In conclusion, IF combined with exercise orchestrates the glycolipid conversion and substrate utilization.

间歇性禁食和运动:协调健康小鼠糖脂转化和利用的双重干预。
间歇性禁食(IF)和运动可以逆转由肥胖引起的糖脂转化能力受损。然而,干扰素和运动结合对正常人糖脂转化能力的影响仍有待确定。在这项研究中,雄性KM小鼠进行了为期6周的IF(包括隔日禁食(ADF),限时禁食(TRF)),跑步机运动,以及两种干预措施的组合。通过检测小鼠血清生化指标及肝脏、腓肠肌和腹股沟脂肪组织中糖脂转化途径密切相关基因和蛋白的表达,探讨两种IF模型联合运动对小鼠糖脂转化的影响。结果表明,IF与运动结合可显著降低小鼠空腹血糖、糖化血清蛋白和肝糖原水平。TRF联合运动可显著降低血清、肝脏和腓肠肌的甘油三酯水平。IF联合运动激活ChREBP或SREBP1/FASN通路,增强葡萄糖介导的脂肪生成通路的转录激活,同时促进脂肪酸氧化蛋白表达,降低肝脏糖异生基因表达,共同提高脂质代谢效率。此外,IF增加了腹沟脂肪组织中糖原转化相关蛋白PPP1R3C和脂肪生热相关蛋白UCP1的表达,表明糖原通量与脂肪生热激活的协调增强。综上所述,IF与运动相结合可以协调糖脂转化和底物利用。
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来源期刊
Molecular and Cellular Biochemistry
Molecular and Cellular Biochemistry 生物-细胞生物学
CiteScore
8.30
自引率
2.30%
发文量
293
审稿时长
1.7 months
期刊介绍: Molecular and Cellular Biochemistry: An International Journal for Chemical Biology in Health and Disease publishes original research papers and short communications in all areas of the biochemical sciences, emphasizing novel findings relevant to the biochemical basis of cellular function and disease processes, as well as the mechanics of action of hormones and chemical agents. Coverage includes membrane transport, receptor mechanism, immune response, secretory processes, and cytoskeletal function, as well as biochemical structure-function relationships in the cell. In addition to the reports of original research, the journal publishes state of the art reviews. Specific subjects covered by Molecular and Cellular Biochemistry include cellular metabolism, cellular pathophysiology, enzymology, ion transport, lipid biochemistry, membrane biochemistry, molecular biology, nuclear structure and function, and protein chemistry.
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