Potential prognostic value and immune landscape of lactylation and liquid-liquid phase separation related genes in acute myeloid leukemia.

IF 1.6 4区医学 Q3 HEMATOLOGY

Hematology Pub Date : 2025-12-01 Epub Date: 2025-09-01 DOI:10.1080/16078454.2025.2548073

Tingxiu Jiang, Hong Chen, Chunjie Qin, Guoran Xie, Qiumei Huang, Shaomei Chen, Yongrong Lai

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引用次数: 0

Abstract

Objectives: Lactylation- and liquid-liquid phase separation-related differentially expressed genes (LLRDEGs) have been implicated in cancer. However, their role in acute myeloid leukemia (AML) remains largely unexplored.

Methods: LLRDEGs associated with AML prognosis were identified using Cox regression and LASSO analyzes. A prognostic model based on three LLRDEGs was constructed for AML, and its associated biological functions were investigated. Furthermore, we evaluated differences in the tumor immune microenvironment based on this prognostic signature.

Results: This study represents the first comprehensive analysis of the prognostic value of LLRDEGs in AML patients, identifying three LLRDEGs with prognostic significance. A prognostic risk model was constructed using these three LLRDEGs, and its prognostic value was validated in an independent external AML dataset. This model was associated with the immune microenvironment of AML. Finally, we observed that chaperonin containing TCP1 subunit 5 (CCT5) was highly expressed in AML. In vitro experiments demonstrated that inhibition of CCT5 induces HL-60 cell cycle arrest and apoptosis.

Conclusion: This research provides a theoretical basis relevant to AML treatment strategies.

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急性髓系白血病中乳酸化和液-液相分离相关基因的潜在预后价值和免疫景观。

目的：乳酸化和液-液相分离相关的差异表达基因（LLRDEGs）与癌症有关。然而，它们在急性髓性白血病（AML）中的作用在很大程度上仍未被探索。方法：采用Cox回归和LASSO分析，鉴定与AML预后相关的LLRDEGs。构建了基于三种llrdeg的AML预后模型，并对其相关生物学功能进行了研究。此外，我们基于这一预后特征评估了肿瘤免疫微环境的差异。结果：本研究首次全面分析了llrdeg在AML患者中的预后价值，确定了3个具有预后意义的llrdeg。使用这三个llrdeg构建了预后风险模型，并在独立的外部AML数据集中验证了其预后价值。该模型与AML的免疫微环境有关。最后，我们观察到含有TCP1亚基5 （CCT5）的伴侣蛋白在AML中高表达。体外实验表明，抑制CCT5可诱导HL-60细胞周期阻滞和凋亡。结论：本研究为AML治疗策略的制定提供了理论依据。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Hematology 医学-血液学

CiteScore

2.60

自引率

5.30%

发文量

140

审稿时长

3 months

期刊介绍： Hematology is an international journal publishing original and review articles in the field of general hematology, including oncology, pathology, biology, clinical research and epidemiology. Of the fixed sections, annotations are accepted on any general or scientific field: technical annotations covering current laboratory practice in general hematology, blood transfusion and clinical trials, and current clinical practice reviews the consensus driven areas of care and management.