Patients with colon cancer exhibited greater TEX19 expression, controlled in vitro by epigenetic inhibitors

IF 2.4 3区生物学 Q2 GENETICS & HEREDITY

Gene Pub Date : 2025-08-31 DOI:10.1016/j.gene.2025.149747

Mikhlid H. Almutairi , Ahmad S. Alkhaldi , Turki M. Alrubie , Waad A. Alsoraie , Jilani P. Shaik , Abdullah M. Alamri , Mohammad Alanazi , Saad H. Alkahtani , Bader O. Almutairi

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引用次数: 0

Abstract

Background

In the Saudi population, colon cancer (CC) is connected with a high death rate due to frequent late-stage diagnosis. Consequently, early diagnosis of CC is crucial for improving treatment outcomes and reducing mortality. The purpose of the study was to evaluate the potential application of human testis expressed 19 (TEX19) as a cancer biomarker for better therapy and early CC identification.

Methods

Both RT-PCR and qRT-PCR were used to examine the expression levels of TEX19 in 25 adjacent CC and normal colon (NC) tissue samples from Saudi male and female patients. To determine if these agents can raise the TEX19, four cell lines treated with 5-aza-2′-deoxycytidine and trichostatin A were subjected to an in vitro investigation using the qRT-PCR technique. Furthermore, a rat model of CC was established using DMH-induced CC in male Wistar rats with saline-treated control colon. Kaplan-Meier survival analysis was performed using The Cancer Genome Atlas (TCGA) dataset.

Results

The qRT-PCR results showed that TEX19 expression was significantly higher in CC compared to NC tissues in both male and female patients (p = 0.0003 and 0.0026, respectively). In vitro experiments demonstrated increased TEX19 expression in cell lines treated with 5-aza-2′-deoxycytidine and in cells treated with trichostatin A. An in vivo rat model also demonstrated increased expression of Tex19.1 and Tex19.2 in DMH (a colon carcinogen)-induced CC compared to the control group. In silico analysis revealed that TEX19 expression was significantly upregulated in COAD tissues compared to normal tissues. TEX19 promoter methylation levels were significantly reduced in COAD tissues compared to normal tissues. Patients with high TEX19 expression exhibited a trend towards poorer overall survival.

Conclusions

These results suggest that the TEX19 gene, which is regulated by the suppression of methylation and histone deacetylation, is a promising biomarker for CC and may serve as a therapeutic target for CC in certain populations.

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结肠癌患者表现出更高的TEX19表达，在体外由表观遗传抑制剂控制。

背景：在沙特人群中，结肠癌（CC）由于频繁的晚期诊断而与高死亡率有关。因此，早期诊断CC对于改善治疗效果和降低死亡率至关重要。该研究的目的是评估人类睾丸表达19 （TEX19）作为癌症生物标志物的潜在应用，以更好地治疗和早期鉴别CC。方法：采用RT-PCR和qRT-PCR方法检测TEX19在沙特男性和女性患者的25例癌旁结肠和正常结肠（NC）组织中的表达水平。为了确定这些药物是否可以提高TEX19，我们使用qRT-PCR技术对4株细胞系进行了5-aza-2'-脱氧胞苷和trichostatin A处理。采用dmh诱导雄性Wistar大鼠结肠盐处理，建立大鼠CC模型。使用癌症基因组图谱（TCGA）数据集进行Kaplan-Meier生存分析。结果：qRT-PCR结果显示，男性和女性CC组织中TEX19的表达均明显高于NC组织（p分别为 = 0.0003和0.0026）。体外实验显示，5-aza-2'-脱氧胞苷处理的细胞系和曲古斯汀a处理的细胞中TEX19的表达增加。与对照组相比，体内大鼠模型中DMH（一种结肠癌致癌物）诱导的CC中Tex19.1和Tex19.2的表达也增加。计算机分析显示，与正常组织相比，TEX19在COAD组织中的表达显著上调。与正常组织相比，COAD组织中TEX19启动子甲基化水平显著降低。TEX19高表达的患者总体生存率较低。结论：这些结果表明，通过抑制甲基化和组蛋白去乙酰化调控的TEX19基因是一种有希望的CC生物标志物，可能作为特定人群CC的治疗靶点。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Gene 生物-遗传学

CiteScore

6.10

自引率

2.90%

发文量

718

审稿时长

42 days

期刊介绍： Gene publishes papers that focus on the regulation, expression, function and evolution of genes in all biological contexts, including all prokaryotic and eukaryotic organisms, as well as viruses.