Technical aspects of loop-mediated isothermal amplification (LAMP) assay in cancer.

IF 3.6 3区 医学 Q1 PATHOLOGY
Nika Asefi, Keivan Majidzadeh-A
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引用次数: 0

Abstract

Introduction: Cancer is a significant health problem worldwide, emphasizing the need for new diagnostic techniques. Among various molecular techniques, loop-mediated isothermal amplification (LAMP) has gained growing interest due to its rapidity, sensitivity, and cost-effectiveness.

Areas covered: This review discusses the use of LAMP to target genes as a biomarker for cancer detection. We performed an extensive literature search to identify relevant oncology studies on LAMP. We highlighted its working principles, advantages over conventional diagnostic methods, and potential limitations in clinical settings.

Expert opinion: LAMP has been reported to be an effective molecular diagnostic technique with tremendous improvements in speed, sensitivity, and affordability. Its potential as a diagnostic tool for cancer detection can provide a viable alternative to conventional diagnostic methods, particularly in low-resource environments. However, challenges such as primer design complexity, possibility of false-positive signals, and standardization issues present hindrances to clinical application. Further research and development are required for further refinement and integration into routine diagnostics. Furthermore, integrating LAMP with other molecular technologies and new platforms may render it increasingly useful in the clinical setting. Continuing research in this field is important for establishing the overall efficacy of LAMP in oncology.

肿瘤中环介导等温扩增(LAMP)测定的技术方面。
导言:癌症是一个全球性的重大健康问题,强调了对新的诊断技术的需求。在各种分子技术中,环介导等温扩增(LAMP)因其快速、敏感和成本效益而受到越来越多的关注。涵盖领域:本文综述了LAMP靶向基因作为癌症检测的生物标志物的应用。我们进行了广泛的文献检索,以确定与LAMP相关的肿瘤学研究。我们强调了它的工作原理,优于传统诊断方法的优势,以及在临床环境中的潜在局限性。专家意见:据报道,LAMP是一种有效的分子诊断技术,在速度、灵敏度和价格上都有很大的提高。它作为癌症检测诊断工具的潜力可以为传统诊断方法提供一种可行的替代方法,特别是在资源匮乏的环境中。然而,引物设计的复杂性、假阳性信号的可能性和标准化问题等挑战阻碍了临床应用。需要进一步的研究和开发以进一步改进和整合到常规诊断中。此外,将LAMP与其他分子技术和新平台相结合,可能会使其在临床环境中越来越有用。该领域的持续研究对于确定LAMP在肿瘤学中的整体疗效至关重要。
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来源期刊
CiteScore
6.60
自引率
0.00%
发文量
71
审稿时长
1 months
期刊介绍: Expert Review of Molecular Diagnostics (ISSN 1473-7159) publishes expert reviews of the latest advancements in the field of molecular diagnostics including the detection and monitoring of the molecular causes of disease that are being translated into groundbreaking diagnostic and prognostic technologies to be used in the clinical diagnostic setting. Each issue of Expert Review of Molecular Diagnostics contains leading reviews on current and emerging topics relating to molecular diagnostics, subject to a rigorous peer review process; editorials discussing contentious issues in the field; diagnostic profiles featuring independent, expert evaluations of diagnostic tests; meeting reports of recent molecular diagnostics conferences and key paper evaluations featuring assessments of significant, recently published articles from specialists in molecular diagnostic therapy. Expert Review of Molecular Diagnostics provides the forum for reporting the critical advances being made in this ever-expanding field, as well as the major challenges ahead in their clinical implementation. The journal delivers this information in concise, at-a-glance article formats: invaluable to a time-constrained community.
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