Enzymatic responses of fish (Ctenopharyngodon idella) exposed to lethal concentrations of selected trace metals.

IF 3.8 3区环境科学与生态学 Q3 ENGINEERING, ENVIRONMENTAL

Environmental Geochemistry and Health Pub Date : 2025-08-22 DOI:10.1007/s10653-025-02718-z

Nazish Shah, Muhammad Khisroon, Said Sajjad Ali Shah, Abdur Rahim

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Abstract

Six sampled tissues were selected to measure their respective enzyme activities through spectrophotometric method. Drastic changes were observed in the enzyme activities of fish under the influence of LC15, LC50 and LC85 copper, chromium and lead administered metals respectively. Significant decrease (p < 0.05) in acetylcholine esterase activity was noted in the metals stressed brain tissue for each of the exposure concentrations as compared to the reference group, whereas among the anti-oxidant enzymes, catalase (CAT) level in stressed liver tissue had a significant increase (p < 0.05) against the respective exposure concentrations. Unlike CAT enzyme level, superoxide dismutase (SOD) in muscle tissue, dropped significantly to nearly negligible level as compared to control group where SOD activity was of maximum level. Since, the levels of enzyme activity effected by the three metals in the entire experimentation were found in a decreasing order of chromium > copper > lead, consequently a prominent level (p < 0.05) of SOD was confirmed at the heaviest exposure concentration (LC85) by chromium during 96 h of time interval. To combat the free radicals produced as result of metals exposure, gluthathione peroxidase activity dramatically increased by nearly 95% as compared to the reference group. Sodium-potassium adenosine triphosphatase (Na + -K + -ATPase) activity in gills significantly decreased in the first metal treated group and returned to the normal levels in the second (LC50) and third treated group (LC85) in case of chromium and copper respectively. Nevertheless, the values obtained for the digestive enzyme pepsin in the fish intestinal tissue had imprecise results.

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鱼暴露于致死浓度的选定微量金属的酶反应。

选取6个样品组织，用分光光度法测定其酶活性。在LC15、LC50和LC85铜、铬和铅的影响下，鱼的酶活性发生了剧烈的变化。显著减少(p铜>铅，因此显著水平(p

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来源期刊

Environmental Geochemistry and Health 环境科学-工程：环境

CiteScore

8.00

自引率

4.80%

发文量

279

审稿时长

4.2 months

期刊介绍： Environmental Geochemistry and Health publishes original research papers and review papers across the broad field of environmental geochemistry. Environmental geochemistry and health establishes and explains links between the natural or disturbed chemical composition of the earth’s surface and the health of plants, animals and people. Beneficial elements regulate or promote enzymatic and hormonal activity whereas other elements may be toxic. Bedrock geochemistry controls the composition of soil and hence that of water and vegetation. Environmental issues, such as pollution, arising from the extraction and use of mineral resources, are discussed. The effects of contaminants introduced into the earth’s geochemical systems are examined. Geochemical surveys of soil, water and plants show how major and trace elements are distributed geographically. Associated epidemiological studies reveal the possibility of causal links between the natural or disturbed geochemical environment and disease. Experimental research illuminates the nature or consequences of natural or disturbed geochemical processes. The journal particularly welcomes novel research linking environmental geochemistry and health issues on such topics as: heavy metals (including mercury), persistent organic pollutants (POPs), and mixed chemicals emitted through human activities, such as uncontrolled recycling of electronic-waste; waste recycling; surface-atmospheric interaction processes (natural and anthropogenic emissions, vertical transport, deposition, and physical-chemical interaction) of gases and aerosols; phytoremediation/restoration of contaminated sites; food contamination and safety; environmental effects of medicines; effects and toxicity of mixed pollutants; speciation of heavy metals/metalloids; effects of mining; disturbed geochemistry from human behavior, natural or man-made hazards; particle and nanoparticle toxicology; risk and the vulnerability of populations, etc.