Centrosomal actin pool levels regulated by localized PKA set the threshold for T cell polarization.

IF 6.2 1区生物学 Q1 BIOCHEMISTRY & MOLECULAR BIOLOGY

EMBO Reports Pub Date : 2025-09-01 Epub Date: 2025-08-26 DOI:10.1038/s44319-025-00533-2

Morgane Simao, Fabienne Régnier, Clotilde Randriamampita

引用次数: 0

Abstract

T lymphocyte migration triggered by chemokine stimulation is preceded by cell polarization. The acquisition of this asymmetry requires a profound cell rearrangement, particularly of the cytoskeleton. The mechanism by which a uniform signal triggered by chemokine receptors rapidly leads to this asymmetry is largely elusive. Using cell imaging, we emphasize that the centrosome dictates the position of the polarization axis in T lymphocytes. Mechanistically, we highlight that the T cell shape is controlled by the amount of actin filaments surrounding the centrosome. In resting conditions as well as after chemokine stimulation, the activity of a specific pool of protein kinase A regulates this cytoskeleton compartment. Once the centrosomal actin is reduced below a certain threshold, the symmetry breaking is catalyzed. This study points to a critical protein kinase A signaling pathway in the establishment of the immune response.

查看原文本刊更多论文

中心体肌动蛋白库水平受局部PKA调控，设定T细胞极化的阈值。

趋化因子刺激触发的T淋巴细胞迁移发生在细胞极化之前。这种不对称性的获得需要深刻的细胞重排，特别是细胞骨架。趋化因子受体触发的统一信号迅速导致这种不对称的机制在很大程度上是难以捉摸的。利用细胞成像，我们强调中心体决定了T淋巴细胞极化轴的位置。在机制上，我们强调T细胞的形状是由围绕着中心体的肌动蛋白丝的数量控制的。在静息状态下以及趋化因子刺激后，一个特定的蛋白激酶a池的活性调节这个细胞骨架室。一旦中心体肌动蛋白减少到一定阈值以下，就会催化对称性断裂。本研究指出了一个关键的蛋白激酶a信号通路在建立免疫应答。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

求助全文

约1分钟内获得全文求助全文

来源期刊

EMBO Reports 生物-生化与分子生物学

CiteScore

11.20

自引率

1.30%

发文量

267

审稿时长

1 months

期刊介绍： EMBO Reports is a scientific journal that specializes in publishing research articles in the fields of molecular biology, cell biology, and developmental biology. The journal is known for its commitment to publishing high-quality, impactful research that provides novel physiological and functional insights. These insights are expected to be supported by robust evidence, with independent lines of inquiry validating the findings. The journal's scope includes both long and short-format papers, catering to different types of research contributions. It values studies that: Communicate major findings: Articles that report significant discoveries or advancements in the understanding of biological processes at the molecular, cellular, and developmental levels. Confirm important findings: Research that validates or supports existing knowledge in the field, reinforcing the reliability of previous studies. Refute prominent claims: Studies that challenge or disprove widely accepted ideas or hypotheses in the biosciences, contributing to the correction and evolution of scientific understanding. Present null data: Papers that report negative results or findings that do not support a particular hypothesis, which are crucial for the scientific process as they help to refine or redirect research efforts. EMBO Reports is dedicated to maintaining high standards of scientific rigor and integrity, ensuring that the research it publishes contributes meaningfully to the advancement of knowledge in the life sciences. By covering a broad spectrum of topics and encouraging the publication of both positive and negative results, the journal plays a vital role in promoting a comprehensive and balanced view of scientific inquiry.