Interference of therapeutic monoclonal antibodies with electrophoresis and immunofixation of serum proteins: state of knowledge and systematic review.

IF 3.7 2区 医学 Q1 MEDICAL LABORATORY TECHNOLOGY
Sacha Pelletier, Laetitia Florent, Philippe Gillery, Jean-Baptiste Oudart
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Abstract

Introduction: The increasing use of therapeutic monoclonal antibodies (t-mAbs) has improved cancer and autoimmune disorder treatment. These therapeutics can interfere with serum protein electrophoresis (SPEP) and immunofixation (IF), potentially leading to the appearance of monoclonal bands that may be misinterpreted as monoclonal gammopathies. Identifying the migration patterns and detection thresholds of t-mAbs is crucial to avoid misinterpretation in clinical laboratories.

Content: A systematic review following PRISMA guidelines was conducted using Pubmed and ScienceDirect databases, with algorithm-based searches and double-blind article selection. Data on the matrix used, separation methods and type of interference were collected into an extraction table.

Summary: A total of 30 articles were included and 30 t-mAbs were described. 11 t-mAbs migrated at the end of the gamma region, 12 in the mid-gamma region, 5 in the early gamma region, one in the beta-2 globulin region and one in the alpha-2 globulin region. Most t-mAbs were detectable by SPEP and IF at concentrations above 100 mg/L.

Outlook: Caution is needed when a new peak appears on SPEP, as it may be mistaken for a monoclonal spike leading to misdiagnosis. Therefore, understanding the migration profiles of these t-mAbs is essential. Different methods are available to remove t-mAbs interference and could be used in daily practice.

用血清蛋白电泳和免疫固定干扰治疗性单克隆抗体:知识现状和系统综述。
越来越多地使用治疗性单克隆抗体(t- mab)改善了癌症和自身免疫性疾病的治疗。这些疗法可能干扰血清蛋白电泳(SPEP)和免疫固定(IF),潜在地导致单克隆带的出现,可能被误解为单克隆伽玛病。确定t- mab的迁移模式和检测阈值对于避免临床实验室的误解至关重要。内容:使用Pubmed和ScienceDirect数据库,采用基于算法的搜索和双盲文章选择,按照PRISMA指南进行系统评价。将所用基质、分离方法和干扰类型的数据收集到提取表中。总结:共纳入了30篇文章,描述了30个 t- mab。11个 t- mab在γ区末端迁移,12个在γ区中期迁移,5个在γ区早期迁移,1个在β -2球蛋白区迁移,1个在α -2球蛋白区迁移。大多数t- mab在浓度大于100 mg/L时可被SPEP和IF检测到。展望:当SPEP上出现新的峰时需要谨慎,因为它可能被误认为单克隆峰导致误诊。因此,了解这些t- mab的迁移特征是必不可少的。有不同的方法可以消除t- mab的干扰,并可用于日常实践。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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来源期刊
Clinical chemistry and laboratory medicine
Clinical chemistry and laboratory medicine 医学-医学实验技术
CiteScore
11.30
自引率
16.20%
发文量
306
审稿时长
3 months
期刊介绍: Clinical Chemistry and Laboratory Medicine (CCLM) publishes articles on novel teaching and training methods applicable to laboratory medicine. CCLM welcomes contributions on the progress in fundamental and applied research and cutting-edge clinical laboratory medicine. It is one of the leading journals in the field, with an impact factor over 3. CCLM is issued monthly, and it is published in print and electronically. CCLM is the official journal of the European Federation of Clinical Chemistry and Laboratory Medicine (EFLM) and publishes regularly EFLM recommendations and news. CCLM is the official journal of the National Societies from Austria (ÖGLMKC); Belgium (RBSLM); Germany (DGKL); Hungary (MLDT); Ireland (ACBI); Italy (SIBioC); Portugal (SPML); and Slovenia (SZKK); and it is affiliated to AACB (Australia) and SFBC (France). Topics: - clinical biochemistry - clinical genomics and molecular biology - clinical haematology and coagulation - clinical immunology and autoimmunity - clinical microbiology - drug monitoring and analysis - evaluation of diagnostic biomarkers - disease-oriented topics (cardiovascular disease, cancer diagnostics, diabetes) - new reagents, instrumentation and technologies - new methodologies - reference materials and methods - reference values and decision limits - quality and safety in laboratory medicine - translational laboratory medicine - clinical metrology Follow @cclm_degruyter on Twitter!
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