Profiling Multiple Steroid Subclasses Enabled by Rounded Turn SLIM Ion Mobility.

IF 2.7 2区 化学 Q2 BIOCHEMICAL RESEARCH METHODS
Cole L Frank, Breland M Jones, Emmaleigh Efird, Makenna Hoover, Selena Kingsley, Heidi M Sabatini, Christopher D Chouinard
{"title":"Profiling Multiple Steroid Subclasses Enabled by Rounded Turn SLIM Ion Mobility.","authors":"Cole L Frank, Breland M Jones, Emmaleigh Efird, Makenna Hoover, Selena Kingsley, Heidi M Sabatini, Christopher D Chouinard","doi":"10.1021/jasms.5c00218","DOIUrl":null,"url":null,"abstract":"<p><p>Ion mobility-mass spectrometry has shown tremendous potential for improving the analysis of various subclasses of steroids. Its speed and ability to separate isobaric and isomeric species makes it ideal for biomedical, clinical, food, environmental, and antidoping analyses. But while other high-resolution ion mobility (HRIM) techniques have begun to see increased use in steroidomics, Structures for Lossless Ion Manipulations (SLIM) is a relative newcomer to the field. HRIM methods are generally challenged with low molecular weight (MW < 400) analysis, but a recently developed SLIM platform has incorporated rounded turns, helium buffer gas, and increased SLIM RF frequency to enable improved low mass transmission. In the present work, we investigate this low mass-tailored SLIM system for the analysis of anabolic androgens, bile acids, endocrine hormones, and ketosteroids. Linear regression-corrected <sup>SLIM</sup>CCS<sub>He</sub> (CCS = collision cross section) values show excellent agreement with values obtained via direct measurement with a drift tube instrument but also underlie the importance of using class- and adduct-specific correction factors. Next, separation performance was evaluated for a range of challenging isomers and demonstrated good resolution for species with ΔCCS of <1%. Finally, the new rounded turn system was compared quantitatively with the traditional 90° (square turn) system in both nitrogen and helium buffer gases and revealed significantly improved signal across the board for rounded turn analyses with helium. Overall, this low mass-tailored system has remarkable promise for development of rapid, targeted steroid analysis in a range of applications.</p>","PeriodicalId":672,"journal":{"name":"Journal of the American Society for Mass Spectrometry","volume":" ","pages":""},"PeriodicalIF":2.7000,"publicationDate":"2025-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Journal of the American Society for Mass Spectrometry","FirstCategoryId":"92","ListUrlMain":"https://doi.org/10.1021/jasms.5c00218","RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q2","JCRName":"BIOCHEMICAL RESEARCH METHODS","Score":null,"Total":0}
引用次数: 0

Abstract

Ion mobility-mass spectrometry has shown tremendous potential for improving the analysis of various subclasses of steroids. Its speed and ability to separate isobaric and isomeric species makes it ideal for biomedical, clinical, food, environmental, and antidoping analyses. But while other high-resolution ion mobility (HRIM) techniques have begun to see increased use in steroidomics, Structures for Lossless Ion Manipulations (SLIM) is a relative newcomer to the field. HRIM methods are generally challenged with low molecular weight (MW < 400) analysis, but a recently developed SLIM platform has incorporated rounded turns, helium buffer gas, and increased SLIM RF frequency to enable improved low mass transmission. In the present work, we investigate this low mass-tailored SLIM system for the analysis of anabolic androgens, bile acids, endocrine hormones, and ketosteroids. Linear regression-corrected SLIMCCSHe (CCS = collision cross section) values show excellent agreement with values obtained via direct measurement with a drift tube instrument but also underlie the importance of using class- and adduct-specific correction factors. Next, separation performance was evaluated for a range of challenging isomers and demonstrated good resolution for species with ΔCCS of <1%. Finally, the new rounded turn system was compared quantitatively with the traditional 90° (square turn) system in both nitrogen and helium buffer gases and revealed significantly improved signal across the board for rounded turn analyses with helium. Overall, this low mass-tailored system has remarkable promise for development of rapid, targeted steroid analysis in a range of applications.

通过圆转弯SLIM离子迁移率分析多个类固醇子类。
离子迁移-质谱法在改进各种类固醇亚类的分析方面显示出巨大的潜力。它的速度和分离等压和同分异构体的能力使其成为生物医学,临床,食品,环境和反兴奋剂分析的理想选择。但是,当其他高分辨率离子迁移(hrm)技术开始在类固醇组学中得到越来越多的应用时,无损离子操纵结构(SLIM)是该领域的一个相对较新的技术。hirm方法通常受到低分子量(MW < 400)分析的挑战,但最近开发的SLIM平台采用了圆形转弯、氦气缓冲气体和增加的SLIM射频频率,以改善低质量传输。在目前的工作中,我们研究了这种低质量定制的SLIM系统,用于分析合成代谢雄激素,胆汁酸,内分泌激素和酮类固醇。线性回归校正的SLIMCCSHe (CCS =碰撞截面)值与漂移管仪器直接测量得到的值非常吻合,但也强调了使用特定类别和加合物校正因子的重要性。接下来,对一系列具有挑战性的异构体的分离性能进行了评估,并证明了具有ΔCCS of的物种具有良好的分辨率
本文章由计算机程序翻译,如有差异,请以英文原文为准。
求助全文
约1分钟内获得全文 求助全文
来源期刊
CiteScore
5.50
自引率
9.40%
发文量
257
审稿时长
1 months
期刊介绍: The Journal of the American Society for Mass Spectrometry presents research papers covering all aspects of mass spectrometry, incorporating coverage of fields of scientific inquiry in which mass spectrometry can play a role. Comprehensive in scope, the journal publishes papers on both fundamentals and applications of mass spectrometry. Fundamental subjects include instrumentation principles, design, and demonstration, structures and chemical properties of gas-phase ions, studies of thermodynamic properties, ion spectroscopy, chemical kinetics, mechanisms of ionization, theories of ion fragmentation, cluster ions, and potential energy surfaces. In addition to full papers, the journal offers Communications, Application Notes, and Accounts and Perspectives
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
确定
请完成安全验证×
copy
已复制链接
快去分享给好友吧!
我知道了
右上角分享
点击右上角分享
0
联系我们:info@booksci.cn Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。 Copyright © 2023 布克学术 All rights reserved.
京ICP备2023020795号-1
ghs 京公网安备 11010802042870号
Book学术文献互助
Book学术文献互助群
群 号:604180095
Book学术官方微信