Evaluation of the Potential Anticonvulsant and Neuroprotective Effects of Ganoderma Lucidum Extract in an Animal Model of Pentylenetetrazol-Induced Seizures

IF 3.8 3区医学 Q2 BIOCHEMISTRY & MOLECULAR BIOLOGY

Neurochemical Research Pub Date : 2025-09-03 DOI:10.1007/s11064-025-04536-2

Itto Rahou Abdessamad, El-Hessni Aboubaker, Bahbiti Youssef, Bikjdaouene Leila, Azeroil Fatima, El Mekhlouf Youssef, Ibouzine-Dine Laila, Mesfioui Abdelhalem

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Abstract

Ganoderma lucidum extract (GLE) has neuro-therapeutic and anticonvulsant effects. This study aimed to evaluate the potential neuroprotective and antioxidant effects of ethanolic extract on pentylenetetrazol-induced brain damage in male Wistar rats and to analyze behavioral manifestations in vivo. Seizure latency, duration, and severity were measured in PTZ-treated rats according to the Racine scale; thus, oxidative stress markers, malondialdehyde (MDA), nitric oxide (NO), and catalase catalytic activity were measured by the 2-thiobarbituric acid, Griess reagent, and hydrogen peroxide methods, respectively. PTZ-injured rats showed both shorter seizure latency and longer seizure duration and intense seizure severity. The effects were enhanced by GLE in a dose-dependent manner. In addition, GLE decreased NO levels, increased catalase levels and decreased MDA levels in the hippocampus and the prefrontal cortex in rats treated with PTZ (75 mg/kg; i.p.). GLE conferred anticonvulsant and neuroprotective effects against PTZ-induced brain damage by repressing molecular processes involved in the production of oxidative stress markers.

Graphical Abstract

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灵芝提取物对戊四唑致癫痫动物模型的潜在抗惊厥和神经保护作用的评价

灵芝提取物（GLE）具有神经治疗和抗惊厥作用。本研究旨在评价乙醇提取物对戊四唑致雄性Wistar大鼠脑损伤的潜在神经保护和抗氧化作用，并分析其在体内的行为表现。根据拉辛量表测量ptz治疗大鼠癫痫发作潜伏期、持续时间和严重程度；因此，氧化应激标志物、丙二醛（MDA）、一氧化氮（NO）和过氧化氢酶的催化活性分别用2-硫代巴比妥酸、Griess试剂和过氧化氢法测定。ptz损伤大鼠发作潜伏期短，发作持续时间长，发作严重程度高。GLE的作用呈剂量依赖性增强。此外，GLE可降低PTZ （75 mg/kg; i.p）大鼠海马和前额叶皮层NO水平、过氧化氢酶水平和MDA水平。GLE通过抑制氧化应激标志物产生的分子过程，对ptz诱导的脑损伤具有抗惊厥和神经保护作用。图形抽象

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来源期刊

Neurochemical Research 医学-神经科学

CiteScore

7.70

自引率

2.30%

发文量

320

审稿时长

6 months

期刊介绍： Neurochemical Research is devoted to the rapid publication of studies that use neurochemical methodology in research on nervous system structure and function. The journal publishes original reports of experimental and clinical research results, perceptive reviews of significant problem areas in the neurosciences, brief comments of a methodological or interpretive nature, and research summaries conducted by leading scientists whose works are not readily available in English.