Prognostic Value of MCM3AP-AS1 in Glioma and its Regulatory Effect on Tumor Progression

IF 3.8 3区医学 Q2 BIOCHEMISTRY & MOLECULAR BIOLOGY

Neurochemical Research Pub Date : 2025-09-04 DOI:10.1007/s11064-025-04539-z

Kang Gao, Lina Song, Deying Niu

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Abstract

Long non-coding RNAs (lncRNAs) have emerged as promising cancer biomarkers due to their stability and detectability. This study aimed to investigate the clinical significance and molecular mechanisms of lncRNA MCA3AP-AS1 in glioma. This study collected the clinical data from 177 glioma patients, and the expression of MCM3AP-AS1 was measured in glioma tissues and cell lines. Kaplan-Meier and COX regression analyses were employed to assess its prognostic value in glioma. In the mechanism study, bioinformatics prediction, correlation analysis, and dual-luciferase assays were conducted to validate the regulatory network involving MCM3AP-AS1, miR-23c, and PIK3R3. Functional experiments (CCK-8, Transwell assays, and Western blot) further determined the impact of MCM3AP-AS1 on glioma cell functions and confirmed the potential regulatory mechanism. Upregulation of MCM3AP-AS1 in glioma was related to the WHO grade, KFS scores, and glioma poor prognosis. Correlation analysis, binding site prediction, and the dual-luciferase reporter assay confirmed the interaction relationship among MCM3AP-AS1, miR-23c, and PIK3R3. In mechanism, MCM3AP-AS1 knockdown suppressed the glioma cell proliferation, migration, invasion, and epithelial-mesenchymal transition (EMT). This attenuated effect of downregulated MCM3AP-AS1 expression in glioma cell proliferation, migration, and invasion could be further reversed by miR-23c inhibition. Upregulated MCM3AP-AS1 expression in glioma was associated with the poor prognosis of glioma. MCM3AP-AS1 may promote glioma progression by enhancing cell proliferation, migration, and invasion through the miR-23c/PIK3R3 axis.

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MCM3AP-AS1在胶质瘤中的预后价值及其对肿瘤进展的调节作用

长链非编码rna （lncRNAs）由于其稳定性和可检测性而成为有前途的癌症生物标志物。本研究旨在探讨lncRNA MCA3AP-AS1在胶质瘤中的临床意义及分子机制。本研究收集了177例胶质瘤患者的临床资料，测定了MCM3AP-AS1在胶质瘤组织和细胞系中的表达。采用Kaplan-Meier和COX回归分析评估其在胶质瘤中的预后价值。在机制研究中，通过生物信息学预测、相关分析和双荧光素酶测定来验证涉及MCM3AP-AS1、miR-23c和PIK3R3的调控网络。功能实验（CCK-8、Transwell实验、Western blot）进一步确定了MCM3AP-AS1对胶质瘤细胞功能的影响，并证实了其潜在的调控机制。MCM3AP-AS1在胶质瘤中的表达上调与WHO分级、KFS评分、胶质瘤不良预后相关。相关分析、结合位点预测和双荧光素酶报告基因检测证实了MCM3AP-AS1、miR-23c和PIK3R3之间的相互作用关系。在机制上，MCM3AP-AS1敲低可抑制胶质瘤细胞的增殖、迁移、侵袭和上皮-间质转化（EMT）。MCM3AP-AS1表达下调对胶质瘤细胞增殖、迁移和侵袭的减弱作用可通过抑制miR-23c进一步逆转。MCM3AP-AS1在胶质瘤中的表达上调与胶质瘤的不良预后相关。MCM3AP-AS1可能通过miR-23c/PIK3R3轴增强细胞增殖、迁移和侵袭，从而促进胶质瘤的进展。

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来源期刊

Neurochemical Research 医学-神经科学

CiteScore

7.70

自引率

2.30%

发文量

320

审稿时长

6 months

期刊介绍： Neurochemical Research is devoted to the rapid publication of studies that use neurochemical methodology in research on nervous system structure and function. The journal publishes original reports of experimental and clinical research results, perceptive reviews of significant problem areas in the neurosciences, brief comments of a methodological or interpretive nature, and research summaries conducted by leading scientists whose works are not readily available in English.