Chronic Waterborne Exposure to Polystyrene Microplastics Induces Kupffer Cell Polarization Imbalance and Hepatic Lipid Accumulation

IF 4.2 2区 生物学 Q2 BIOCHEMISTRY & MOLECULAR BIOLOGY
Ting Li, Zhenghong Yao, Yuxin Huang, Ran Li, Lu Zhang, Rucheng Chen, Weijia Gu
{"title":"Chronic Waterborne Exposure to Polystyrene Microplastics Induces Kupffer Cell Polarization Imbalance and Hepatic Lipid Accumulation","authors":"Ting Li,&nbsp;Zhenghong Yao,&nbsp;Yuxin Huang,&nbsp;Ran Li,&nbsp;Lu Zhang,&nbsp;Rucheng Chen,&nbsp;Weijia Gu","doi":"10.1096/fj.202500910RR","DOIUrl":null,"url":null,"abstract":"<p>Microplastics (MPs), particles under 5 mm, are widespread environmental contaminants. Polystyrene (PS), used in many household items, degrades into polystyrene MPs (PS-MPs), which accumulate in the environment. Chronic exposure to waterborne PS-MPs was found to disrupt hepatic lipid metabolism in C57BL/6N mice through inflammatory Kupffer cell polarization and IL-17/NF-κB signaling pathways. While short-term PS-MPs exposure revealed preferential accumulation in the liver and testes, long-term exposure (9–12 weeks) induced significant increases in body fat percentage and hepatic lipid deposition independent of dietary changes. Mechanistically, chronic PS-MPs exposure promoted Kupffer cell polarization toward pro-inflammatory M1 phenotypes, accompanied by upregulated IL-17 expression and suppressed anti-inflammatory cytokines. Western blot analysis demonstrated concurrent elevation of lipid synthesis markers with reduced lipid oxidation and transport proteins. These findings established that PS-MPs accumulation drives hepatic steatosis through dual mechanisms of macrophage-mediated inflammation and impaired lipid homeostasis pathways.</p>","PeriodicalId":50455,"journal":{"name":"The FASEB Journal","volume":"39 17","pages":""},"PeriodicalIF":4.2000,"publicationDate":"2025-09-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://faseb.onlinelibrary.wiley.com/doi/epdf/10.1096/fj.202500910RR","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"The FASEB Journal","FirstCategoryId":"99","ListUrlMain":"https://faseb.onlinelibrary.wiley.com/doi/10.1096/fj.202500910RR","RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q2","JCRName":"BIOCHEMISTRY & MOLECULAR BIOLOGY","Score":null,"Total":0}
引用次数: 0

Abstract

Microplastics (MPs), particles under 5 mm, are widespread environmental contaminants. Polystyrene (PS), used in many household items, degrades into polystyrene MPs (PS-MPs), which accumulate in the environment. Chronic exposure to waterborne PS-MPs was found to disrupt hepatic lipid metabolism in C57BL/6N mice through inflammatory Kupffer cell polarization and IL-17/NF-κB signaling pathways. While short-term PS-MPs exposure revealed preferential accumulation in the liver and testes, long-term exposure (9–12 weeks) induced significant increases in body fat percentage and hepatic lipid deposition independent of dietary changes. Mechanistically, chronic PS-MPs exposure promoted Kupffer cell polarization toward pro-inflammatory M1 phenotypes, accompanied by upregulated IL-17 expression and suppressed anti-inflammatory cytokines. Western blot analysis demonstrated concurrent elevation of lipid synthesis markers with reduced lipid oxidation and transport proteins. These findings established that PS-MPs accumulation drives hepatic steatosis through dual mechanisms of macrophage-mediated inflammation and impaired lipid homeostasis pathways.

Abstract Image

Abstract Image

Abstract Image

慢性水接触聚苯乙烯微塑料诱导库普弗细胞极化失衡和肝脏脂质积累
微塑料(MPs), 5毫米以下的颗粒,是广泛存在的环境污染物。聚苯乙烯(PS),用于许多家庭用品,降解成聚苯乙烯MPs (PS-MPs),在环境中积累。发现长期暴露于水性PS-MPs可通过炎性Kupffer细胞极化和IL-17/NF-κB信号通路破坏C57BL/6N小鼠肝脏脂质代谢。PS-MPs短期暴露在肝脏和睾丸中优先积累,而长期暴露(9-12周)会导致体脂率和肝脏脂质沉积显著增加,而不受饮食变化的影响。从机制上讲,慢性PS-MPs暴露促进Kupffer细胞向促炎M1表型极化,并伴有IL-17表达上调和抗炎细胞因子抑制。Western blot分析显示脂质合成标记物同时升高,脂质氧化和转运蛋白减少。这些发现表明,PS-MPs积累通过巨噬细胞介导的炎症和脂质稳态受损途径的双重机制驱动肝脏脂肪变性。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
求助全文
约1分钟内获得全文 求助全文
来源期刊
The FASEB Journal
The FASEB Journal 生物-生化与分子生物学
CiteScore
9.20
自引率
2.10%
发文量
6243
审稿时长
3 months
期刊介绍: The FASEB Journal publishes international, transdisciplinary research covering all fields of biology at every level of organization: atomic, molecular, cell, tissue, organ, organismic and population. While the journal strives to include research that cuts across the biological sciences, it also considers submissions that lie within one field, but may have implications for other fields as well. The journal seeks to publish basic and translational research, but also welcomes reports of pre-clinical and early clinical research. In addition to research, review, and hypothesis submissions, The FASEB Journal also seeks perspectives, commentaries, book reviews, and similar content related to the life sciences in its Up Front section.
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
确定
请完成安全验证×
copy
已复制链接
快去分享给好友吧!
我知道了
右上角分享
点击右上角分享
0
联系我们:info@booksci.cn Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。 Copyright © 2023 布克学术 All rights reserved.
京ICP备2023020795号-1
ghs 京公网安备 11010802042870号
Book学术文献互助
Book学术文献互助群
群 号:604180095
Book学术官方微信