Hanneda A. Fomukong, Idowu A. Aimola, Abubakar Sani, Mamman Mohammed, Halima Bello-Manga, Gloria Chechet, Joy U. Ameloko, Reuben S. Baba, Abduljabar Musa, Pecular Nwanyibunwa Okoro
求助PDF
{"title":"Optimized Enrichment of CD71+ Reticulocytes From Whole Blood for Single-Cell RNA Sequencing","authors":"Hanneda A. Fomukong, Idowu A. Aimola, Abubakar Sani, Mamman Mohammed, Halima Bello-Manga, Gloria Chechet, Joy U. Ameloko, Reuben S. Baba, Abduljabar Musa, Pecular Nwanyibunwa Okoro","doi":"10.1002/cpz1.70203","DOIUrl":null,"url":null,"abstract":"<p>Reticulocytes are a group of immature red blood cells (RBCs) produced in the bone marrow and released into the bloodstream where they mature into RBCs within 1 to 2 days. They play a crucial role in assessing bone health and many hematological diseases. Isolating viable reticulocytes for use in single-cell transcriptomics has been a challenge due to their similarity to mature RBCs. Their low concentration and continuous maturation to RBCs with time also poses a great challenge. In this article, we optimized a method for enrichment of CD71<sup>+</sup> reticulocytes from whole blood, with a high yield and viability for downstream studies. © 2025 Wiley Periodicals LLC.</p><p><b>Basic Protocol 1</b>: Blood tissue preparation and reticulocyte enrichment</p><p><b>Basic Protocol 2</b>: Visualization and determination of cell viability</p><p><b>Alternate Protocol</b>: Counting cells using a hemocytometer</p>","PeriodicalId":93970,"journal":{"name":"Current protocols","volume":"5 9","pages":""},"PeriodicalIF":2.2000,"publicationDate":"2025-09-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Current protocols","FirstCategoryId":"1085","ListUrlMain":"https://currentprotocols.onlinelibrary.wiley.com/doi/10.1002/cpz1.70203","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
引用次数: 0
引用
批量引用
Abstract
Reticulocytes are a group of immature red blood cells (RBCs) produced in the bone marrow and released into the bloodstream where they mature into RBCs within 1 to 2 days. They play a crucial role in assessing bone health and many hematological diseases. Isolating viable reticulocytes for use in single-cell transcriptomics has been a challenge due to their similarity to mature RBCs. Their low concentration and continuous maturation to RBCs with time also poses a great challenge. In this article, we optimized a method for enrichment of CD71+ reticulocytes from whole blood, with a high yield and viability for downstream studies. © 2025 Wiley Periodicals LLC.
Basic Protocol 1 : Blood tissue preparation and reticulocyte enrichment
Basic Protocol 2 : Visualization and determination of cell viability
Alternate Protocol : Counting cells using a hemocytometer
全血CD71+网织红细胞的优化富集用于单细胞RNA测序
网织红细胞是一组未成熟的红细胞（红细胞），产生于骨髓，释放到血液中，在1至2天内成熟为红细胞。它们在评估骨骼健康和许多血液系统疾病中起着至关重要的作用。由于网织红细胞与成熟红细胞的相似性，分离网织红细胞用于单细胞转录组学一直是一个挑战。它们的低浓度和随着时间的推移对红细胞的不断成熟也构成了很大的挑战。在本文中，我们优化了一种从全血中富集CD71+网织红细胞的方法，该方法具有高产率和下游研究的可行性。©2025 Wiley期刊有限公司基本方案1：血液组织制备和网织红细胞富集基本方案2：细胞活力的可视化和测定备用方案：使用血细胞计计数细胞
本文章由计算机程序翻译，如有差异，请以英文原文为准。
求助内容：
应助结果提醒方式：
京公网安备 11010802042870号
