Active Humidity Control Chamber for Desorption Electrospray Ionization-Mass Spectrometry Imaging Applications

IF 2.7 2区 化学 Q2 BIOCHEMICAL RESEARCH METHODS
Hawkins S. Shepard, Robert L. G. Gottschalk, Jody C. May and John A. McLean*, 
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引用次数: 0

Abstract

Ambient ionization techniques enable mass spectrometry (MS) to expand into broader experimental contexts, although it is increasingly clear that results are influenced by the environmental conditions at the site of sampling. Desorption electrospray ionization (DESI), in particular, is affected by variations in relative humidity (RH) levels. Here we describe the design, development, and construction of an environmental control chassis that can actively modulate RH within ± 3% of user-defined set points across a broad humidity range (15%–70% RH). Preliminary characterization demonstrated differential analyte responses across a range of set points, with observed enhancement of leucine-enkephalin, sulfadimethoxine (negative mode), and maltose (positive mode) in response to increased humidity. The measurable differences in analyte signals across discrete humidity set points underscore the importance of environmental control in ambient ionization strategies. The humidity control system outlined here can be translated to other DESI platforms, with construction information provided herein.

主动湿度控制室解吸电喷雾电离质谱成像应用
环境电离技术使质谱法(MS)能够扩展到更广泛的实验环境中,尽管越来越清楚的是,结果受到采样地点环境条件的影响。特别是,解吸电喷雾电离(DESI)受到相对湿度(RH)水平变化的影响。在这里,我们描述了一种环境控制机箱的设计、开发和构造,该机箱可以在广泛的湿度范围(15%-70% RH)内,在用户自定义设定点的±3%范围内主动调节RH。初步表征显示了不同分析物在一系列设定值上的反应,观察到亮氨酸-脑啡肽、磺胺二甲氧嘧啶(阴性模式)和麦芽糖(阳性模式)对湿度增加的反应增强。分析物信号在离散湿度设定点上的可测量差异强调了环境电离策略中环境控制的重要性。这里概述的湿度控制系统可以转换为其他DESI平台,并提供施工信息。
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来源期刊
CiteScore
5.50
自引率
9.40%
发文量
257
审稿时长
1 months
期刊介绍: The Journal of the American Society for Mass Spectrometry presents research papers covering all aspects of mass spectrometry, incorporating coverage of fields of scientific inquiry in which mass spectrometry can play a role. Comprehensive in scope, the journal publishes papers on both fundamentals and applications of mass spectrometry. Fundamental subjects include instrumentation principles, design, and demonstration, structures and chemical properties of gas-phase ions, studies of thermodynamic properties, ion spectroscopy, chemical kinetics, mechanisms of ionization, theories of ion fragmentation, cluster ions, and potential energy surfaces. In addition to full papers, the journal offers Communications, Application Notes, and Accounts and Perspectives
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