Effect of human amniotic membrane on the consolidation during bone lengthening by distraction: Experimental study in rabbits

IF 1.4 4区医学 Q3 ORTHOPEDICS

Journal of Orthopaedic Science Pub Date : 2025-09-01 DOI:10.1016/j.jos.2024.11.011

Najah Elmounedi , Nizar Sahnoun , Ahmed Racem Guidara , Walid Bahloul , Khaled Keskes , Zoubaier Ellouz , Walid Elbaya , Hassib Keskes

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引用次数: 0

Abstract

Objective

This study aimed to assess the effect of implantation of fresh human amniotic membranes (HAM) on bone consolidation during distraction bone lengthening.

Methods

Ten New Zealand white rabbits were used in this study. For each rabbit, we performed a diaphyseal tibial osteotomy after installing a single-plane distraction external fixator. Rabbits were divided into 2 groups (n = 5): an osteotomy and rapid lengthening of 2.8 mm/days (negative control (NC) group) or an osteotomy with implantation of fresh HAM and rapid lengthening of 2.8 mm/days (HAM group). We carried out a rapid lengthening at a rate of 2 turns/day for 7 days and a single turn on the 8th day knowing that one turn corresponds to an elongation of 1.4 mm. The total elongation obtained was 21 mm corresponding to 20–25 % of the length of the rabbit's tibia. A radiological study was carried out on 4 and 8 weeks after the end of lengthening. Micro-computed tomography (μ-CT) analysis was carried out 8 weeks after the end of the lengthening and after the sacrifice of the rabbits associated with histological analyses.

Results

For the HAM group, radiographic analysis showed that all cases consolidated with a homogeneous callus visible at 1 month after lengthening, and complete corticalization was demonstrated at μ-CT analysis. Histological staining exhibited thick continuous cortices with intramedullary fibro adipose tissue with a medulla rich in hematopoietic cells.

Conclusion

The results show that HAM affects the stimulation of bone consolidation during bone lengthening by distraction.

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人羊膜在兔牵张骨延长过程中固结作用的实验研究

目的探讨新鲜人羊膜（HAM）植入对牵张骨延长术中骨巩固的影响。方法选用新西兰大白兔10只。对于每只兔子，我们在安装单平面牵引外固定架后进行了骨干胫骨截骨。将家兔分为2组（n = 5）：截骨快速延长2.8 mm/d（阴性对照（NC）组）和截骨植入新鲜火腿快速延长2.8 mm/d （HAM组）。我们以2转/天的速度进行了7天的快速延长，并在第8天进行了一次延长，因为我们知道1转对应于1.4 mm的延长。获得的总伸长率为21 mm，相当于兔胫骨长度的20 - 25%。延长结束后4周和8周进行影像学检查。显微计算机断层扫描（μ-CT）分析于延长结束后8周和兔的牺牲后进行，并进行组织学分析。结果HAM组的x线分析显示，所有病例在延长1个月后均可见均匀的愈伤组织，μ-CT分析显示完全皮质化。组织学染色显示厚的连续皮层，髓内纤维脂肪组织，髓质富含造血细胞。结论骨牵张延长术中HAM对骨巩固的刺激有一定影响。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Journal of Orthopaedic Science 医学-整形外科

CiteScore

3.00

自引率

0.00%

发文量

290

审稿时长

90 days

期刊介绍： The Journal of Orthopaedic Science is the official peer-reviewed journal of the Japanese Orthopaedic Association. The journal publishes the latest researches and topical debates in all fields of clinical and experimental orthopaedics, including musculoskeletal medicine, sports medicine, locomotive syndrome, trauma, paediatrics, oncology and biomaterials, as well as basic researches.