Genetic Enhancement of Sugar Recovery Percentage in Commercial Sugarcane by Inducing Sweet Sucrose Isomerase Gene III (SSGIII)

IF 2 3区 农林科学 Q2 AGRONOMY
Mudassar Fareed Awan, Usman Arif, Muhammad Farhan Sarwar, Momna Toqeer Dar, Safia Obaidur Rab
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Abstract

Enhancing the sugar yield in sugarcane is considered a huge challenge due to the inherent complexity of its metabolic pathways and genomic organization. The incorporation of novel isomerase enzymes encoded through the Sweet Sucrose Isomerase Gene III (SSGIII) emerged as a promising strategy to augment sugar recovery percentage. This study meticulously engineered the SSGIII, optimized for expression in sugarcane, and subsequently introduced it into plant expression vectors under the influence of single and double promoter systems in conjunction with vacuole-targeted signal peptides at the 3′ end. The real-time PCR (RT-PCR) manifested the increased expression of SSGIII in sugarcane stalks relative to the leaves recommending a tissue-specific enhancement of sugar metabolism. The tested transgenic lines, SS-70, SS-55, SS-111, upheld very high level of sweetness in sugarcane. Transgenic line SS-220 transformed with triple gene constructs exhibited the maximum level of enhancement in sugar contents with a 27% improvement than13% over control line. This unprecedented augmentation was also seen to increase with maturity while reached optimum at the age of 18 months, underscoring the developmental dynamics influencing gene efficacy. The elite sugarcane line SS-220 displayed the highest Isomaltulose concentration (650 mM), substantiating the potential of triple gene constructs for maximal metabolic redirection. Furthermore, the Brix analysis corroborated these findings, exhibiting a surge in sugar content encompassing over 100% in transgenic lines relative to control plants. This investigation highlights the transformation potential of leveraging tailored genetic modifications, through the strategic deployment of SSGIII under diverse promoter systems, to significantly elevate sugar content in sugarcane. These advancements not only enhance the economic viability of sugarcane cultivation but also pave the way for future biotechnological interventions in crop improvement.

诱导蔗糖异构酶III基因提高商品甘蔗糖回收率的遗传研究
由于甘蔗代谢途径和基因组组织的内在复杂性,提高甘蔗的糖产量被认为是一个巨大的挑战。通过甜蔗糖异构酶基因III (SSGIII)编码的新型异构酶的掺入成为提高糖回收率的一种有希望的策略。本研究精心设计了SSGIII,优化了其在甘蔗中的表达,随后在单启动子和双启动子系统的影响下,结合液泡靶向信号肽在3 '端将其引入植物表达载体。实时荧光定量PCR (RT-PCR)结果显示,甘蔗茎秆中SSGIII的表达相对于叶片有所增加,表明糖代谢的组织特异性增强。测试的转基因品系SS-70、SS-55、SS-111在甘蔗中保持了很高的甜度。经三重基因构建的转基因品系SS-220的糖含量比对照品系提高了27%,比对照品系提高了13%。这种前所未有的增强也随着成熟而增加,在18个月时达到最佳,强调了影响基因功效的发育动力学。优质甘蔗系SS-220表现出最高的异麦芽糖浓度(650 mM),证实了三重基因构建具有最大代谢重定向的潜力。此外,Brix的分析证实了这些发现,显示相对于对照植物,转基因品系的糖含量激增超过100%。该研究强调了利用定制遗传修饰的转化潜力,通过在不同启动子系统下战略性地部署SSGIII,显著提高甘蔗的糖含量。这些进步不仅提高了甘蔗种植的经济可行性,而且为未来生物技术干预作物改良铺平了道路。
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来源期刊
Sugar Tech
Sugar Tech AGRONOMY-
CiteScore
3.90
自引率
21.10%
发文量
145
期刊介绍: The journal Sugar Tech is planned with every aim and objectives to provide a high-profile and updated research publications, comments and reviews on the most innovative, original and rigorous development in agriculture technologies for better crop improvement and production of sugar crops (sugarcane, sugar beet, sweet sorghum, Stevia, palm sugar, etc), sugar processing, bioethanol production, bioenergy, value addition and by-products. Inter-disciplinary studies of fundamental problems on the subjects are also given high priority. Thus, in addition to its full length and short papers on original research, the journal also covers regular feature articles, reviews, comments, scientific correspondence, etc.
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