Behavioral and systemic differences in Holstein cows with known severity of rumen acidosis during low and high-grain feeding

IF 2 2区农林科学 Q1 AGRICULTURE, DAIRY & ANIMAL SCIENCE

Applied Animal Behaviour Science Pub Date : 2025-08-28 DOI:10.1016/j.applanim.2025.106800

Rana Muhammad Atif , Patrick Biber , Thomas Hartinger , Ezequias Castillo-Lopez , Nicole Reisinger , Qendrim Zebeli

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引用次数: 0

Abstract

This study investigated behavioral and systemic differences in second-lactation Holstein cows with known susceptibility to subacute ruminal acidosis (SARA) when transitioned from a low to high-grain diet. Eighteen cows (9 SARA-susceptible, 9 SARA-resistant; 646 ± 59 kg body weight) were used in a longitudinal experimental design, with two experimental runs. In each run, the cows were first fed a 40 % concentrate diet for 2 weeks followed by a 65 % concentrate diet for 4 weeks. Behavioral parameters (chewing, lying, eating, ruminating, feed sorting), salivary characteristics, milk production, and blood metabolites were evaluated. SARA-resistant cows exhibited greater rumination activity (rumination time, chews per bolus, chews per minute; P < 0.05), higher body weight (P = 0.04), and elevated serum total protein (P = 0.01), despite no differences in ruminal pH indices. The dietary shift to high-grain significantly affected most behavioral, production, and physiological parameters (P < 0.01), including increased sorting for physically effective fiber (peNDF), eating rate, meal frequency, milk yield, milk protein, blood glucose, and salivary osmolality, alongside reductions in rumination time, eating duration, milk fat, and blood lipid metabolites. Significant SARA type × diet interactions were observed in rumination indices, eating behavior (visit size, number of meals), blood glucose, and non-esterified fatty acids concentrations, with SARA-resistant cows showing improved metabolic adaptation during the high-grain phase. In conclusion, cows previously identified as SARA-resistant demonstrated enhanced behavioral and metabolic resilience to high-grain diets, suggesting that prior SARA status may influence adaptation strategies during dietary transitions. Additional research is suggested to evaluate prolonged impact of high-grain diets on behavioral and production parameters across different SARA phenotypes.

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低粒和高粒饲养中已知严重程度瘤胃酸中毒的荷斯坦奶牛的行为和全身差异

本研究研究了已知易患亚急性瘤胃酸中毒（SARA）的第二泌乳荷斯坦奶牛从低粒饲粮过渡到高粒饲粮时的行为和全身差异。采用纵向试验设计，选取18头奶牛（9头易感，9头耐药，体重646 ± 59 kg），进行2期试验。每轮犊牛先饲喂40% %精料2周，再饲喂65% %精料4周。行为参数（咀嚼、躺卧、进食、反刍、饲料分类）、唾液特征、产奶量和血液代谢物被评估。抗sara奶牛反刍活动（反刍时间、每丸咀嚼量、每分钟咀嚼量；P <； 0.05）、体重增加（P = 0.04）、血清总蛋白升高（P = 0.01），但瘤胃pH指数无显著差异。饲粮转向高谷物显著影响了大多数行为、生产和生理参数（P <； 0.01），包括对物理有效纤维（peNDF）的分选增加、进食率、进餐频率、产奶量、乳蛋白、血糖和唾液渗透压，以及反刍时间、进食时间、乳脂和血脂代谢产物的减少。在反刍指标、进食行为（访食量、餐数）、血糖和非酯化脂肪酸浓度方面，SARA型与 日粮的交互作用显著，SARA抗性奶牛在高粒期表现出更好的代谢适应。综上所述，先前被鉴定为SARA抗性的奶牛对高谷物日粮表现出更强的行为和代谢恢复能力，这表明先前的SARA状态可能会影响饮食转换期间的适应策略。建议进一步研究高谷物日粮对不同SARA表型的行为和生产参数的长期影响。

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来源期刊

Applied Animal Behaviour Science 农林科学-行为科学

CiteScore

4.40

自引率

21.70%

发文量

191

审稿时长

18.1 weeks

期刊介绍： This journal publishes relevant information on the behaviour of domesticated and utilized animals. Topics covered include: -Behaviour of farm, zoo and laboratory animals in relation to animal management and welfare -Behaviour of companion animals in relation to behavioural problems, for example, in relation to the training of dogs for different purposes, in relation to behavioural problems -Studies of the behaviour of wild animals when these studies are relevant from an applied perspective, for example in relation to wildlife management, pest management or nature conservation -Methodological studies within relevant fields The principal subjects are farm, companion and laboratory animals, including, of course, poultry. The journal also deals with the following animal subjects: -Those involved in any farming system, e.g. deer, rabbits and fur-bearing animals -Those in ANY form of confinement, e.g. zoos, safari parks and other forms of display -Feral animals, and any animal species which impinge on farming operations, e.g. as causes of loss or damage -Species used for hunting, recreation etc. may also be considered as acceptable subjects in some instances -Laboratory animals, if the material relates to their behavioural requirements