Inhibition of USP46/ANK2 Axis Alleviates Myocardial Infarction in Hypoxia/Reoxygenation-Treated Cardiomyocytes and Ischemia/Reperfusion-Induced Rat Models
Zhong Xie, Jiming Zhou, Dan Huang, Wei Luo, Junbi Zhao
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Abstract
Ankyrin2 (ANK2) has been found to be abnormally overexpressed in myocardial infarction (MI) cell models, but its role and related mechanisms in MI progression have not been explored. Cardiomyocytes (AC16) were subjected to hypoxia/reoxygenation (H/R) treatment to mimic MI cell models, and ischemia/reperfusion (I/R)-induced myocardial injury was used to establish MI rat models. Cell viability, apoptosis and mitochondrial membrane potential (MMP) depolarization were analyzed by CCK8 assay, flow cytometry and JC-1 staining. The levels of inflammatory-related factors and ferroptosis-related markers were determined by commercial kits. ANK2 and ubiquitin-specific peptidase 46 (USP46) protein levels were analyzed using western blot. Cycloheximide treatment assay and ubiquitination assay were used to confirm the regulation of USP46 on ANK2 protein stability and ubiquitination level. After H/R treatment, AC16 cell viability was reduced, while apoptosis, inflammation and ferroptosis were enhanced. ANK2 was upregulated in H/R-induced AC16 cells, and its knockdown repressed H/R-induced cardiomyocyte apoptosis, inflammation and ferroptosis. USP46 enhanced ANK2 protein stability and expression by deubiquitination. Overexpression of ANK2 also reversed the inhibition effect of USP46 knockdown on H/R-induced cardiomyocyte injury. In addition, downregulation of USP46 alleviated myocardial injury in I/R-induced rat models by decreasing ANK2 expression. USP46-stabilized ANK2 promoted cardiomyocyte apoptosis, inflammation and ferroptosis to aggravate MI process.
期刊介绍：
The Journal of Biochemical and Molecular Toxicology is an international journal that contains original research papers, rapid communications, mini-reviews, and book reviews, all focusing on the molecular mechanisms of action and detoxication of exogenous and endogenous chemicals and toxic agents. The scope includes effects on the organism at all stages of development, on organ systems, tissues, and cells as well as on enzymes, receptors, hormones, and genes. The biochemical and molecular aspects of uptake, transport, storage, excretion, lactivation and detoxication of drugs, agricultural, industrial and environmental chemicals, natural products and food additives are all subjects suitable for publication. Of particular interest are aspects of molecular biology related to biochemical toxicology. These include studies of the expression of genes related to detoxication and activation enzymes, toxicants with modes of action involving effects on nucleic acids, gene expression and protein synthesis, and the toxicity of products derived from biotechnology.
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