Response of primary mammary epithelial cells to pathogen challenge in dairy cows with divergent genomic breeding values for udder health

IF 3 2区生物学 Q2 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Genomics Pub Date : 2025-08-26 DOI:10.1016/j.ygeno.2025.111102

Terhi Iso-Touru , Daniel Fischer , Frank Panitz , Suvi Taponen , Zexi Cai , Goutam Sahana , Ilma Tapio , Johanna Vilkki

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Abstract

This study focuses on the initial cellular response to a causal pathogen in the mammary gland of dairy cows aiming to uncover genomic features associated with mastitis resistance. We analyzed transcriptomes from Escherichia coli -challenged primary bovine mammary epithelial cells derived from milk of cows with high or low genomic index value for udder health. The most striking difference was the markedly higher number of differentially expressed genes post-challenge in the low mastitis resistance group. Gene enrichment analysis indicates a slightly delayed adaptive immune response and early modification of primary metabolic processes in the low mastitis resistance group. Notably, there was no overlap between the DEGs or their potential cis-eQTL with the location of candidate SNPs from association studies of the same cow population. This implies that the genes and pathways involved in the early immune response in the mammary epithelium may not be central for phenotypic mastitis resistance.

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奶牛原代乳腺上皮细胞对病原菌攻击的响应

本研究的重点是奶牛乳腺中对因果病原体的初始细胞反应，旨在揭示与乳腺炎抗性相关的基因组特征。我们分析了大肠杆菌感染的原代牛乳腺上皮细胞的转录组，这些细胞来自乳腺健康基因组指数高或低的奶牛的牛奶。最显著的差异是在低乳腺炎抵抗组中，攻毒后差异表达基因的数量明显增加。基因富集分析表明，低乳腺炎抵抗组的适应性免疫反应轻微延迟，初级代谢过程早期改变。值得注意的是，在同一奶牛群体的关联研究中，deg或其潜在的顺式eqtl与候选snp的位置没有重叠。这意味着参与乳腺上皮早期免疫反应的基因和途径可能不是表型乳腺炎抵抗的中心。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Genomics 生物-生物工程与应用微生物

CiteScore

9.60

自引率

2.30%

发文量

260

审稿时长

60 days

期刊介绍： Genomics is a forum for describing the development of genome-scale technologies and their application to all areas of biological investigation. As a journal that has evolved with the field that carries its name, Genomics focuses on the development and application of cutting-edge methods, addressing fundamental questions with potential interest to a wide audience. Our aim is to publish the highest quality research and to provide authors with rapid, fair and accurate review and publication of manuscripts falling within our scope.