First report of nodularin production by Nostochopsis sp. in a temperate eutrophic lake

IF 4.5 1区生物学 Q1 MARINE & FRESHWATER BIOLOGY

Harmful Algae Pub Date : 2025-08-19 DOI:10.1016/j.hal.2025.102956

Abby M. Webster , Zac Triumph , Bofan Wei , Robbie M. Martin , Laura E. Smith , Steven W. Wilhelm , Lisa B. Cleckner , N.Roxanna Razavi , Gregory L. Boyer

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Abstract

Benthic cyanobacteria are understudied in comparison to their planktonic counterparts. Consequently, our understanding of cyanotoxin production in benthic mats remains limited. We detected nodularin-R (NOD-R) in a population of Nostochopsis from Honeoye Lake (New York, United States). Identification as Nostochopsis was supported by morphological (see section 3.1 for a detailed description) and metagenomic analyses. The first metagenome assembled genome of Nostochopsis was drafted and estimated at 99.28 % complete. Cyanotoxin testing of Nostochopsis biomass showed production of NOD-R but not microcystins, anatoxins, or cylindrospermopsins. The complete nodularin synthetase cluster was confirmed in the Nostochopsis genome. To our knowledge, this is the first report of nodularin production, or any cyanotoxin production, by the benthic cyanobacteria Nostochopsis. This is the fourth genus of cyanobacteria reported to produce nodularins.

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温带富营养化湖泊Nostochopsis sp.产生结核素的首次报道

与浮游生物相比，底栖蓝藻的研究不足。因此，我们对底栖垫中蓝藻毒素产生的了解仍然有限。我们在来自美国纽约Honeoye Lake的Nostochopsis种群中检测到了结节素r （nodarin - r）。形态学（详细描述见3.1节）和宏基因组学分析支持了该菌株的Nostochopsis鉴定。绘制了首个Nostochopsis元基因组组装基因组，估计完成度为99.28%。蓝藻毒素测试表明，蓝藻生物量产生NOD-R，但不产生微囊藻毒素、anatoxins或圆筒精子蛋白酶。完整的结核素合成酶簇在结核杆菌基因组中得到证实。据我们所知，这是第一个报告结核素生产，或任何蓝藻毒素生产，由底栖蓝藻菌Nostochopsis。这是第四个据报道产生结核杆菌的蓝藻属。

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来源期刊

Harmful Algae 生物-海洋与淡水生物学

CiteScore

12.50

自引率

15.20%

发文量

122

审稿时长

7.5 months

期刊介绍： This journal provides a forum to promote knowledge of harmful microalgae and macroalgae, including cyanobacteria, as well as monitoring, management and control of these organisms.