Protective Potential of Hispidulin Against Oleic Acid-Induced Acute Kidney Injury: Investigation of Oxidative Stress, Inflammation and Cellular Death Mechanisms

IF 2.8 3区 医学 Q2 BIOCHEMISTRY & MOLECULAR BIOLOGY
Bahri Avci, Handan Uguz, Esra Palabiyik, Ayse Nurseli Sulumer, Hakan Askin
{"title":"Protective Potential of Hispidulin Against Oleic Acid-Induced Acute Kidney Injury: Investigation of Oxidative Stress, Inflammation and Cellular Death Mechanisms","authors":"Bahri Avci,&nbsp;Handan Uguz,&nbsp;Esra Palabiyik,&nbsp;Ayse Nurseli Sulumer,&nbsp;Hakan Askin","doi":"10.1002/jbt.70461","DOIUrl":null,"url":null,"abstract":"<div>\n \n <p>Hispidulin is a natural flavonoid extracted from many plants such as <i>Saussurea involucrata</i> by different methods. The present study aims to evaluate the histopathologic, antioxidant and molecular effects of hispidulin in oleic acid-induced male <i>Spraque Dawley</i> rats. Accordingly, rats were divided into three separate groups HC: Healthy Control Group OA: Oleic Acid Group and H + OA Group. Oxidative stress markers superoxide dismutase (SOD), catalase (CAT), glutathione (GSH), malondialdehyde (MDA) and myeloperoxidase (MPO) were evaluated in kidney tissues obtained from the animals of the treatment groups. The same tissues were subjected to histopathologic examination. Moreover, gene expression levels of critical regulators of apoptosis, cellular metabolism and inflammation were examined by real-time PCR to identify the molecular structure responsible for the nephrotoxic impact of oleic acid. It was observed that oleic acid (OA) led to decrease in GSH level and an increase in MPO and MDA levels in rat kidney tissues. The levels of SOD and CAT, which are among the antioxidant system components, were found to decrease. In addition, kidney damage biomarker (<i>Kim-1</i>), inflammation genes <i>(Il-6</i>, <i>Nf-Kß</i>), apoptotic gene (<i>Casp3</i>) and gene involved in extracellular matrix renewal (<i>Mmp2</i>) were negatively affected by OA exposure. However, it was observed that hispidulin was able to reverse all the deregulations induced by OA administration. In conclusion, oleic acid, used as an inducer of acute kidney injury, caused tissue damage by disrupting the oxidant and antioxidant balance and dysfunction in renal tissues by increasing the levels of inflammatory mediators. Hispidulin, which we use as a renoprotective agent in acute kidney injury, may be considered as an alternative, versatile and effective technique to alleviate renal injury by inhibiting inflammation, tubular cell death and oxidative stress.</p></div>","PeriodicalId":15151,"journal":{"name":"Journal of Biochemical and Molecular Toxicology","volume":"39 9","pages":""},"PeriodicalIF":2.8000,"publicationDate":"2025-08-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Journal of Biochemical and Molecular Toxicology","FirstCategoryId":"3","ListUrlMain":"https://onlinelibrary.wiley.com/doi/10.1002/jbt.70461","RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q2","JCRName":"BIOCHEMISTRY & MOLECULAR BIOLOGY","Score":null,"Total":0}
引用次数: 0

Abstract

Hispidulin is a natural flavonoid extracted from many plants such as Saussurea involucrata by different methods. The present study aims to evaluate the histopathologic, antioxidant and molecular effects of hispidulin in oleic acid-induced male Spraque Dawley rats. Accordingly, rats were divided into three separate groups HC: Healthy Control Group OA: Oleic Acid Group and H + OA Group. Oxidative stress markers superoxide dismutase (SOD), catalase (CAT), glutathione (GSH), malondialdehyde (MDA) and myeloperoxidase (MPO) were evaluated in kidney tissues obtained from the animals of the treatment groups. The same tissues were subjected to histopathologic examination. Moreover, gene expression levels of critical regulators of apoptosis, cellular metabolism and inflammation were examined by real-time PCR to identify the molecular structure responsible for the nephrotoxic impact of oleic acid. It was observed that oleic acid (OA) led to decrease in GSH level and an increase in MPO and MDA levels in rat kidney tissues. The levels of SOD and CAT, which are among the antioxidant system components, were found to decrease. In addition, kidney damage biomarker (Kim-1), inflammation genes (Il-6, Nf-Kß), apoptotic gene (Casp3) and gene involved in extracellular matrix renewal (Mmp2) were negatively affected by OA exposure. However, it was observed that hispidulin was able to reverse all the deregulations induced by OA administration. In conclusion, oleic acid, used as an inducer of acute kidney injury, caused tissue damage by disrupting the oxidant and antioxidant balance and dysfunction in renal tissues by increasing the levels of inflammatory mediators. Hispidulin, which we use as a renoprotective agent in acute kidney injury, may be considered as an alternative, versatile and effective technique to alleviate renal injury by inhibiting inflammation, tubular cell death and oxidative stress.

Abstract Image

Hispidulin对油酸引起的急性肾损伤的保护潜力:氧化应激、炎症和细胞死亡机制的研究
Hispidulin是一种从雪莲等多种植物中通过不同方法提取的天然类黄酮。本研究旨在探讨海鞘磷脂对油酸诱导的雄性大鼠的组织病理学、抗氧化和分子作用。将大鼠分为健康对照组、油酸组和H + OA组。测定各处理组动物肾组织中氧化应激标志物超氧化物歧化酶(SOD)、过氧化氢酶(CAT)、谷胱甘肽(GSH)、丙二醛(MDA)和髓过氧化物酶(MPO)的含量。对相同组织进行组织病理学检查。此外,通过实时PCR检测细胞凋亡、细胞代谢和炎症关键调控因子的基因表达水平,以确定油酸对肾毒性影响的分子结构。结果表明,油酸(OA)可导致大鼠肾组织GSH水平降低,MPO和MDA水平升高。研究发现,抗氧化系统中SOD和CAT的水平下降。此外,肾损伤生物标志物(Kim-1)、炎症基因(Il-6、Nf-Kß)、凋亡基因(Casp3)和参与细胞外基质更新的基因(Mmp2)均受到OA暴露的负面影响。然而,观察到hispidulin能够逆转OA引起的所有失调。综上所述,油酸作为急性肾损伤的诱导剂,通过增加炎症介质的水平,破坏肾组织的氧化和抗氧化平衡,造成组织损伤。Hispidulin作为急性肾损伤的肾保护剂,可以被认为是一种替代的、通用的、有效的技术,通过抑制炎症、小管细胞死亡和氧化应激来减轻肾损伤。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
求助全文
约1分钟内获得全文 求助全文
来源期刊
CiteScore
5.80
自引率
2.80%
发文量
277
审稿时长
6-12 weeks
期刊介绍: The Journal of Biochemical and Molecular Toxicology is an international journal that contains original research papers, rapid communications, mini-reviews, and book reviews, all focusing on the molecular mechanisms of action and detoxication of exogenous and endogenous chemicals and toxic agents. The scope includes effects on the organism at all stages of development, on organ systems, tissues, and cells as well as on enzymes, receptors, hormones, and genes. The biochemical and molecular aspects of uptake, transport, storage, excretion, lactivation and detoxication of drugs, agricultural, industrial and environmental chemicals, natural products and food additives are all subjects suitable for publication. Of particular interest are aspects of molecular biology related to biochemical toxicology. These include studies of the expression of genes related to detoxication and activation enzymes, toxicants with modes of action involving effects on nucleic acids, gene expression and protein synthesis, and the toxicity of products derived from biotechnology.
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
确定
请完成安全验证×
copy
已复制链接
快去分享给好友吧!
我知道了
右上角分享
点击右上角分享
0
联系我们:info@booksci.cn Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。 Copyright © 2023 布克学术 All rights reserved.
京ICP备2023020795号-1
ghs 京公网安备 11010802042870号
Book学术文献互助
Book学术文献互助群
群 号:604180095
Book学术官方微信