Ultrasound-assisted extraction with water of lyophilized camu-camu (Myrciaria dubia) resulted in high antioxidant and antimicrobial inhibition

IF 2.4 4区生物学 Q3 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Journal of chemical technology and biotechnology Pub Date : 2025-07-11 DOI:10.1002/jctb.70013

Evelyn Diane Pereira, Daniel Vianey Cardoso, Edson Antônio da Silva, Carina Contini Triques, Erica Camila Pavan, Kátia Andressa Santos, Ricardo Fiori Zara, Jaqueline Hoscheid, Márcia Regina Fagundes-Klen

{"title":"Ultrasound-assisted extraction with water of lyophilized camu-camu (Myrciaria dubia) resulted in high antioxidant and antimicrobial inhibition","authors":"Evelyn Diane Pereira, Daniel Vianey Cardoso, Edson Antônio da Silva, Carina Contini Triques, Erica Camila Pavan, Kátia Andressa Santos, Ricardo Fiori Zara, Jaqueline Hoscheid, Márcia Regina Fagundes-Klen","doi":"10.1002/jctb.70013","DOIUrl":null,"url":null,"abstract":"<div>\n \n \n <section>\n \n <h3> BACKGROUND</h3>\n \n <p>In this study, lyophilized camu-camu (<i>Myrciaria dubia</i>) fruits were subjected to ultrasound-assisted extraction (UAE) using water, ammonia solution pH 9.5, and hexane. Each solvent was evaluated following a 2<sup>3</sup> factorial experimental design to assess the effects of amplitude, solvent-to-solid ratio, and temperature on extract yield and antioxidant potential. The results were compared to those obtained by the conventional method of dynamic maceration (DM). The UAE times were 20 min for water and ammonia solution pH 9.5, and 30 min for hexane.</p>\n </section>\n \n <section>\n \n <h3> RESULTS</h3>\n \n <p>The highest yield (47.78%) was reached with an amplitude of 90% for water, a solid-to-sample ratio of 22 mL g<sup>−1</sup>, and a temperature of 35 °C for water. On average, UAE yielded approximately 26% more extract than DM, requiring only one-tenth of the extraction time, resulting in significantly lower energy consumption. Although all extracts exhibited antioxidant activity, the aqueous extract showed the highest value (3056 μmol TE·g<sup>−1</sup>). Furthermore, it demonstrated stronger antimicrobial activity than ascorbic acid. High concentrations of ascorbic acid and phenolic compounds were also detected in the aqueous extract.</p>\n </section>\n \n <section>\n \n <h3> CONCLUSION</h3>\n \n <p>When using water as a solvent, the best results were achieved enabling the comparison among different polarities and pH values. This result is advantageous from an economic and environmental point of view. In addition, the characteristics that the extract expressed showed that it can be used as a nutraceutical or even in the food industry, enabling an added value to the extract from this raw material. © 2025 Society of Chemical Industry (SCI).</p>\n </section>\n </div>","PeriodicalId":15335,"journal":{"name":"Journal of chemical technology and biotechnology","volume":"100 9","pages":"1956-1972"},"PeriodicalIF":2.4000,"publicationDate":"2025-07-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Journal of chemical technology and biotechnology","FirstCategoryId":"5","ListUrlMain":"https://scijournals.onlinelibrary.wiley.com/doi/10.1002/jctb.70013","RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q3","JCRName":"BIOTECHNOLOGY & APPLIED MICROBIOLOGY","Score":null,"Total":0}

引用次数: 0

Abstract

BACKGROUND

In this study, lyophilized camu-camu (Myrciaria dubia) fruits were subjected to ultrasound-assisted extraction (UAE) using water, ammonia solution pH 9.5, and hexane. Each solvent was evaluated following a 2³ factorial experimental design to assess the effects of amplitude, solvent-to-solid ratio, and temperature on extract yield and antioxidant potential. The results were compared to those obtained by the conventional method of dynamic maceration (DM). The UAE times were 20 min for water and ammonia solution pH 9.5, and 30 min for hexane.

RESULTS

The highest yield (47.78%) was reached with an amplitude of 90% for water, a solid-to-sample ratio of 22 mL g⁻¹, and a temperature of 35 °C for water. On average, UAE yielded approximately 26% more extract than DM, requiring only one-tenth of the extraction time, resulting in significantly lower energy consumption. Although all extracts exhibited antioxidant activity, the aqueous extract showed the highest value (3056 μmol TE·g⁻¹). Furthermore, it demonstrated stronger antimicrobial activity than ascorbic acid. High concentrations of ascorbic acid and phenolic compounds were also detected in the aqueous extract.

CONCLUSION

When using water as a solvent, the best results were achieved enabling the comparison among different polarities and pH values. This result is advantageous from an economic and environmental point of view. In addition, the characteristics that the extract expressed showed that it can be used as a nutraceutical or even in the food industry, enabling an added value to the extract from this raw material. © 2025 Society of Chemical Industry (SCI).

Abstract Image

查看原文本刊更多论文

超声波辅助提取双歧桃金娘（Myrciaria dubia）的冻干水具有较高的抗氧化和抑菌活性

本研究采用水、pH为9.5的氨溶液和己烷对冻干的卡姆（Myrciaria dubia）果实进行超声辅助提取。采用23因子实验设计对每种溶剂进行评估，以评估振幅、溶剂固比和温度对提取物得率和抗氧化潜力的影响。结果与常规动态浸渍法（DM）的结果进行了比较。水和氨溶液pH为9.5时，UAE时间为20 min，己烷为30 min。结果在固料比为22 mL g−1，水处理温度为35℃，水处理振幅为90%时，收率最高，为47.78%。平均而言，阿联酋的提取量比DM多出约26%，只需要十分之一的提取时间，从而显著降低了能耗。所有提取物均表现出抗氧化活性，其中水提液的抗氧化活性最高（3056 μmol TE·g−1）。此外，它还显示出比抗坏血酸更强的抗菌活性。在水提取物中也检测到高浓度的抗坏血酸和酚类化合物。结论以水为溶剂，可比较不同极性和pH值，效果最佳。从经济和环境的角度来看，这一结果是有利的。此外，提取物所表现出的特性表明，它可以用作营养保健品，甚至可以用于食品工业，从而使这种原料的提取物具有附加值。©2025化学工业学会（SCI）。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

求助全文

约1分钟内获得全文求助全文

来源期刊

Journal of chemical technology and biotechnology 工程技术-工程：化工

CiteScore

7.00

自引率

5.90%

发文量

268

审稿时长

1.7 months

期刊介绍： Journal of Chemical Technology and Biotechnology(JCTB) is an international, inter-disciplinary peer-reviewed journal concerned with the application of scientific discoveries and advancements in chemical and biological technology that aim towards economically and environmentally sustainable industrial processes.