Analysis of APOD and FABP7 genes polymorphisms and their association with feed efficiency in Hu sheep

IF 1.8 3区农林科学 Q2 AGRICULTURE, DAIRY & ANIMAL SCIENCE

Small Ruminant Research Pub Date : 2025-11-01 Epub Date: 2025-08-20 DOI:10.1016/j.smallrumres.2025.107601

Ziyue Xiao , Deyin Zhang , YuKun Zhang , Dan Xu , Zongwu Ma , Kunchao Han , Lijuan He , Chengqi Yan , Qi Zhang , Lianjun Feng , Mengru Pu , Lei Gao , Peiliang Cao , Guoxing Jia , Dewen Kong , Linting Li , Jian Zhang , Hongjian Li , Jianlin Wang , Fadi Li , Huibin Tian

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引用次数: 0

Abstract

Feed efficiency is a critical determinant of feeding costs in sheep production, and its improvement can significantly enhance economic returns, and fat deposition is an important factor affecting feed efficiency. The Apolipoprotein D (APOD) gene influences fat formation and regulates lipid metabolism. The Fatty Acid Binding Proteins 7 (FABP7) gene is associated with fatty acid synthesis and transport. This study aimed to investigate the expression patterns and polymorphisms of the APOD and FABP7 genes in Hu sheep and their associations with feed efficiency traits, thereby identifying novel candidate molecular markers for selective breeding. A population of 1010 Hu sheep was used to determine the expression levels of APOD and FABP7 genes in 10 tissues using real-time quantitative PCR (qRT-PCR). SNP loci were identified via Sanger sequencing, followed by genotyping through an Allele-Specific Quantitative PCR based genotyping assay (AQP). The associations between these genotypes and feed efficiency traits were subsequently analyzed. The results indicated that the APOD gene had the highest expression level in tail fat, while the lowest expression level in spleen; The FABP7 gene was highly expressed in rumen, and the lowest expression level in muscle. Synonymous mutation was found in exons at APOD SNP (Chr1:192889132 T > C) and intron mutation was found at FABP7 SNP (Chr8:15690488 A>G). The association analysis indicated that both APOD SNP (Chr1:192889132 T > C) and FABP7 SNP (Chr8:15690488 A>G) were significantly associated with average daily weight gain (ADG) and feed conversion ratio (FCR) (P < 0.05) of the Hu sheep in the 120–180 d growth phase. Furthermore, in the combined genotype association analysis, these loci also showed a significant association with the FCR (P < 0.05). These findings suggest that polymorphisms in the APOD and FABP7 loci may be useful as molecular markers to enhance feed efficiency in Hu sheep.

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湖羊APOD和FABP7基因多态性分析及其与饲料效率的关系

饲料效率是绵羊生产中决定饲养成本的关键因素，提高饲料效率可显著提高经济效益，而脂肪沉积是影响饲料效率的重要因素。载脂蛋白D （APOD）基因影响脂肪形成并调节脂质代谢。脂肪酸结合蛋白7 （FABP7）基因与脂肪酸的合成和运输有关。本研究旨在研究湖羊APOD和FABP7基因的表达模式、多态性及其与饲料效率性状的关系，为选择育种寻找新的候选分子标记。采用实时荧光定量PCR （real-time quantitative PCR, qRT-PCR）技术，测定了1010只湖羊10个组织中APOD和FABP7基因的表达水平。通过Sanger测序确定SNP位点，然后通过基于等位基因特异性定量PCR的基因分型试验（AQP）进行基因分型。随后分析了这些基因型与饲料效率性状之间的关系。结果表明，APOD基因在尾脂肪中表达量最高，在脾脏中表达量最低；FABP7基因在瘤胃中表达量最高，在肌肉中表达量最低。在APOD SNP （Chr1:192889132 T >； C）和FABP7 SNP （Chr8:15690488 A>；G）外显子中发现同源突变。关联分析表明，APOD SNP （Chr1:192889132 T >； C）和FABP7 SNP （Chr8:15690488 A>；G）与120 ~ 180 d生长期湖羊的平均日增重（ADG）和饲料系数（FCR）（P <； 0.05）显著相关。此外，在联合基因型关联分析中，这些位点也显示出与FCR显著相关（P <； 0.05）。上述结果提示，APOD和FABP7基因座的多态性可作为提高湖羊饲料效率的分子标记。

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来源期刊

Small Ruminant Research 农林科学-奶制品与动物科学

CiteScore

3.10

自引率

11.10%

发文量

210

审稿时长

12.5 weeks

期刊介绍： Small Ruminant Research publishes original, basic and applied research articles, technical notes, and review articles on research relating to goats, sheep, deer, the New World camelids llama, alpaca, vicuna and guanaco, and the Old World camels. Topics covered include nutrition, physiology, anatomy, genetics, microbiology, ethology, product technology, socio-economics, management, sustainability and environment, veterinary medicine and husbandry engineering.