Protective effect of exosomes derived from platelet-rich plasma during human sperm cryopreservation.

IF 3.7 2区生物学 Q3 CELL BIOLOGY

Molecular and Cellular Biochemistry Pub Date : 2025-08-19 DOI:10.1007/s11010-025-05371-3

Fatemeh Karimi, Marzieh Kafami, Fatemeh Ghodrati Khakestar, Mohammad Hossein Madahali, Samad Nazemi, Sareh Karimi

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引用次数: 0

Abstract

Fertility preservation is a critical aspect of improving human life quality. Sperm cryopreservation is highly recommended for men at risk of infertility; however, it can have detrimental effects on sperm quality. In this study, we evaluate the effects of platelet-rich plasma (PRP) and PRP-derived exosomes on cryopreservation outcomes in men with asthenoteratozoospermia referred to a public infertility center. The semen samples from 75 men with asthenoteratozoospermia (based on WHO 2020 criteria) were randomly divided into five groups: Control (conventional freezing), Exo/before, Exo/After, PRP/Before, and PRP/After. In all intervention groups, PRP or exosomes were added either 1 h before freezing or 1 h after thawing. Sperm parameters and oxidative stress were evaluated in all samples. Sperm incubation with exosomes before freezing significantly improves motility and lowers DNA fragmentation index (DFI) and oxidative stress compared to the control and other groups. Based on the present study, semen incubation with exosomes for 1 h before freezing not only protects sperm against oxidative stress but also enhances some sperm parameters, such as motility, in men with asthenoteratozoospermia.

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富血小板血浆外泌体在人类精子冷冻保存中的保护作用。

保存生育能力是提高人类生活质量的一个重要方面。精子冷冻保存被强烈推荐给有不育风险的男性；然而，它会对精子质量产生不利影响。在这项研究中，我们评估了富血小板血浆（PRP）和PRP来源的外泌体对到公共不育中心就诊的男性弱异精子症患者冷冻保存结果的影响。将75例弱异精子症男性的精液样本（根据WHO 2020标准）随机分为5组：对照组（常规冷冻）、Exo/before、Exo/After、PRP/ before和PRP/After。在所有干预组中，PRP或外泌体在冷冻前1 h或解冻后1 h加入。对所有样本的精子参数和氧化应激进行评估。与对照组和其他组相比，冷冻前与外泌体孵育的精子显著提高了活力，降低了DNA断裂指数（DFI）和氧化应激。根据本研究，精子在冷冻前与外泌体孵育1小时，不仅可以保护精子免受氧化应激，还可以提高精子的一些参数，如活力。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Molecular and Cellular Biochemistry 生物-细胞生物学

CiteScore

8.30

自引率

2.30%

发文量

293

审稿时长

1.7 months

期刊介绍： Molecular and Cellular Biochemistry: An International Journal for Chemical Biology in Health and Disease publishes original research papers and short communications in all areas of the biochemical sciences, emphasizing novel findings relevant to the biochemical basis of cellular function and disease processes, as well as the mechanics of action of hormones and chemical agents. Coverage includes membrane transport, receptor mechanism, immune response, secretory processes, and cytoskeletal function, as well as biochemical structure-function relationships in the cell. In addition to the reports of original research, the journal publishes state of the art reviews. Specific subjects covered by Molecular and Cellular Biochemistry include cellular metabolism, cellular pathophysiology, enzymology, ion transport, lipid biochemistry, membrane biochemistry, molecular biology, nuclear structure and function, and protein chemistry.