Ultrasonography-assisted near-infrared spectroscopy imaging enhanced by gold nanorods in a xenograft mouse model of prostate cancer.

IF 2.5 3区医学 Q2 RADIOLOGY, NUCLEAR MEDICINE & MEDICAL IMAGING

Ultrasonography Pub Date : 2025-09-01 Epub Date: 2025-07-08 DOI:10.14366/usg.25072

Seungsoo Lee, Dae Chul Jung, Seung-Seob Kim, Jaemoon Yang, Yoochan Hong, Dalkwon Koh

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Abstract

Purpose: This study aimed to develop and evaluate a near-infrared spectroscopy (NIRS) system enhanced by gold nanorods (GNRs) for the detection of prostate cancer using phantom and xenograft mouse models.

Methods: A hybrid ultrasound-NIRS (US-NIRS) system was created with a 785 nm wavelength, integrating eight laser diodes and four detectors with a linear ultrasound probe. Software for processing near-infrared (NIR) signals was developed using an engineering toolkit and an image reconstruction package. Two optical phantoms simulating prostate cancer were constructed using TiO2 for scattering effects and India ink for absorption effects, each containing a cylindrical cavity for GNRs positioned at depths of 1 cm and 2 cm. A xenograft mouse model was prepared by injecting PC-3 cells into the right flank of nude mice. PEGylated GNRs (GNR-PEG) were synthesized. US-NIRS imaging was performed on mice before and after intravenous injection of GNR-PEG.

Results: Ultrasonography revealed solid, vascular tumors without necrosis or hemorrhage. Preinjection NIRS showed higher baseline NIR absorbance in tumors compared to normal tissue (optical depths: 0.26, 1.52, and 0.24 for the 1.5 cm, 1.4 cm, and 0.5 cm tumors, respectively). After GNR-PEG injection, tumor optical depths significantly increased (3.36, 4.39, and 1.69 for the 1.5 cm, 1.4 cm, and 0.5 cm tumors, respectively), peaking around 5 minutes, and subsequently decreasing towards baseline levels by 60 minutes.

Conclusion: A US-NIRS hybrid imaging system enhanced by GNR-PEG demonstrated increased NIR absorption in prostate cancer xenografts. This fusion imaging technique holds potential for future clinical applications in detecting prostate cancer.

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超声辅助近红外光谱成像金纳米棒增强异种前列腺癌小鼠模型。

目的：建立并评价金纳米棒（GNRs）增强近红外光谱（NIRS）检测前列腺癌模型和异种移植小鼠模型。方法：建立超声-近红外光谱（US-NIRS）混合系统，该系统波长为785 nm，由8个激光二极管和4个探测器与线性超声探头组成。使用工程工具包和图像重建包开发了处理近红外（NIR）信号的软件。利用TiO2散射效应和印度墨水吸收效应构建了两个模拟前列腺癌的光学幻影，每个幻影都包含一个圆柱形腔，位于1 cm和2 cm的深度，用于gnr。将PC-3细胞注入裸鼠右侧，制备异种移植小鼠模型。合成了聚乙二醇化gnr （GNR-PEG）。小鼠静脉注射GNR-PEG前后进行US-NIRS成像。结果：超声示实性血管性肿瘤，无坏死、出血。注射前的近红外光谱显示，与正常组织相比，肿瘤的基线近红外吸光度更高（1.5 cm、1.4 cm和0.5 cm肿瘤的光学深度分别为0.26、1.52和0.24）。注射GNR-PEG后，肿瘤光学深度显著增加（1.5 cm、1.4 cm和0.5 cm的肿瘤分别为3.36、4.39和1.69），在5分钟左右达到峰值，随后向基线水平下降60分钟。结论：GNR-PEG增强的US-NIRS混合成像系统显示前列腺癌异种移植物对NIR的吸收增加。这种融合成像技术在检测前列腺癌方面具有潜在的临床应用前景。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Ultrasonography Medicine-Radiology, Nuclear Medicine and Imaging

CiteScore

5.10

自引率

6.50%

发文量

审稿时长

15 weeks

期刊介绍： Ultrasonography, the official English-language journal of the Korean Society of Ultrasound in Medicine (KSUM), is an international peer-reviewed academic journal dedicated to practice, research, technology, and education dealing with medical ultrasound. It is renamed from the Journal of Korean Society of Ultrasound in Medicine in January 2014, and published four times per year: January 1, April 1, July 1, and October 1. Original articles, technical notes, topical reviews, perspectives, pictorial essays, and timely editorial materials are published in Ultrasonography covering state-of-the-art content. Ultrasonography aims to provide updated information on new diagnostic concepts and technical developments, including experimental animal studies using new equipment in addition to well-designed reviews of contemporary issues in patient care. Along with running KSUM Open, the annual international congress of KSUM, Ultrasonography also serves as a medium for cooperation among physicians and specialists from around the world who are focusing on various ultrasound technology and disease problems and relevant basic science.