Qiutong Lu, Zhaopeng Wang, Shuixian Cao, Huan Wang, Nianshuang Li, Yi Hu, Wuhui Ding, Wei Zuo, Junbo Hong
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引用次数: 0
Abstract
Helicobacter pylori (H. pylori) infection is a significant cause of gastric diseases, with its pathogenic mechanisms still not fully understood. This study investigates the role of METTL3, an enzyme involved in m6A methylation, in modulating the CXCL1/NF-κB signaling pathway in H. pylori-induced gastritis. Using both bioinformatics analysis of GEO database and experimental approaches including MeRIP, RIP assays, and immunostaining, this research highlights how METTL3 influences CXCL1 expression and NF-κB pathway activation. Results from both in vitro and in vivo models show that METTL3 increases inflammatory responses and apoptosis in gastric cells. Suppression of METTL3 resulted in decreased inflammation and apoptosis, suggesting its potential as a therapeutic target in gastritis management.
幽门螺杆菌（Helicobacter pylori， H. pylori）感染是胃部疾病的重要病因，其致病机制尚不完全清楚。本研究探讨了METTL3（一种参与m6A甲基化的酶）在幽门螺杆菌诱导的胃炎中调节CXCL1/NF-κB信号通路的作用。本研究利用GEO数据库的生物信息学分析和MeRIP、RIP和免疫染色等实验方法，重点研究了METTL3如何影响CXCL1表达和NF-κB通路激活。体外和体内模型的结果表明，METTL3增加了胃细胞的炎症反应和凋亡。抑制METTL3导致炎症和细胞凋亡减少，提示其作为胃炎治疗靶点的潜力。
期刊介绍：
Brought to readers by the editorial team of Cell Death & Differentiation, Cell Death & Disease is an online peer-reviewed journal specializing in translational cell death research. It covers a wide range of topics in experimental and internal medicine, including cancer, immunity, neuroscience, and now cancer metabolism.
Cell Death & Disease seeks to encompass the breadth of translational implications of cell death, and topics of particular concentration will include, but are not limited to, the following:
Experimental medicine
Cancer
Immunity
Internal medicine
Neuroscience
Cancer metabolism
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