Refining Zooplankton Diet Composition Studies Over Short and Long Time Scales by Combining 18S Metabarcoding With Fatty Acid Analyses.

IF 5.5 1区 生物学 Q1 BIOCHEMISTRY & MOLECULAR BIOLOGY
Nora-Charlotte Pauli, Katja Metfies, Stefan Neuhaus, Martin Graeve, Alison C Cleary, Morten H Iversen, Bettina Meyer
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引用次数: 0

Abstract

Understanding diet composition is essential for unravelling trophic interactions in aquatic ecosystems. DNA metabarcoding, utilising various variable regions of the 18S rRNA gene, is increasingly employed to investigate zooplankton diet composition. However, accurate results depend on rapid inactivation of digestive enzymes and DNA nucleases through proper sample processing and preservation. In this study, we compare the prey communities of Antarctic krill retrieved from the 18S variable regions V4 and V7 and assess how different processing treatments affect the detected prey composition of both krill and salps. Our findings highlight the critical importance of prompt sample processing for species with highly efficient digestive enzymes, such as krill, to preserve rapidly digested prey, including gelatinous plankton. Comparative analyses of the V4 and V7 regions revealed significantly different prey communities within the same krill samples, indicating that these regions may not be suitable for direct comparisons within or across studies. To complement molecular approaches, we also analyse fatty acids (FA) as trophic markers which provide insights into dietary habits over both short and long time scales. By comparing FA signals from stomach and tissue samples of the same krill and salp individuals, we identified significant differences in trophic markers representing different plankton groups. These findings emphasise the necessity of separating digestive tract from tissue to distinguish between short- and long-term diet signals. Furthermore, integrating FA analysis with metabarcoding offers valuable insights into zooplankton digestion efficiency across taxonomic levels. This combined approach enhances our understanding of zooplankton feeding ecology and trophic interactions in marine ecosystems.

结合18S元条形码和脂肪酸分析,改进浮游动物饮食组成在短期和长期尺度上的研究。
了解饮食组成对于揭示水生生态系统中的营养相互作用至关重要。DNA元条形码利用18S rRNA基因的各种可变区域,越来越多地用于研究浮游动物的饮食组成。然而,准确的结果取决于通过适当的样品处理和保存,使消化酶和DNA核酸酶迅速失活。本研究比较了从18S可变区V4和V7采集到的南极磷虾的猎物群落,评估了不同处理方式对磷虾和海带探测到的猎物组成的影响。我们的研究结果强调了对具有高效消化酶的物种(如磷虾)进行快速样品处理的重要性,以保存快速消化的猎物,包括凝胶状浮游生物。V4和V7区域的对比分析显示,同一磷虾样本中猎物群落存在显著差异,表明这些区域可能不适合在研究内部或跨研究进行直接比较。为了补充分子方法,我们还分析了脂肪酸(FA)作为营养标志物,它提供了短期和长期饮食习惯的见解。通过比较同一磷虾和salp个体的胃和组织样本的FA信号,我们发现了代表不同浮游生物群体的营养标志物的显著差异。这些发现强调了将消化道从组织中分离出来以区分短期和长期饮食信号的必要性。此外,将FA分析与元条形码相结合,可以跨分类水平对浮游动物消化效率提供有价值的见解。这种综合方法增强了我们对海洋生态系统中浮游动物摄食生态学和营养相互作用的理解。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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来源期刊
Molecular Ecology Resources
Molecular Ecology Resources 生物-进化生物学
CiteScore
15.60
自引率
5.20%
发文量
170
审稿时长
3 months
期刊介绍: Molecular Ecology Resources promotes the creation of comprehensive resources for the scientific community, encompassing computer programs, statistical and molecular advancements, and a diverse array of molecular tools. Serving as a conduit for disseminating these resources, the journal targets a broad audience of researchers in the fields of evolution, ecology, and conservation. Articles in Molecular Ecology Resources are crafted to support investigations tackling significant questions within these disciplines. In addition to original resource articles, Molecular Ecology Resources features Reviews, Opinions, and Comments relevant to the field. The journal also periodically releases Special Issues focusing on resource development within specific areas.
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