Innovative therapeutic modalities of purified platelet-derived growth factors on PDX1, Neurog3, and renin expressions in STZ-induced diabetic nephropathy and pancreatic injury

IF 5.1 2区医学 Q1 MEDICINE, RESEARCH & EXPERIMENTAL

Life sciences Pub Date : 2025-08-18 DOI:10.1016/j.lfs.2025.123921

Mohamed Elzallat , Marwa Hassan , Hossam M. Fahmy , Eman El-Ahwany , Nagwan M. Salama , Hoda Abu-Taleb , Tarek Aboushousha , Mahmoud Balata , Dina Mostafa Mohammed

{"title":"Innovative therapeutic modalities of purified platelet-derived growth factors on PDX1, Neurog3, and renin expressions in STZ-induced diabetic nephropathy and pancreatic injury","authors":"Mohamed Elzallat , Marwa Hassan , Hossam M. Fahmy , Eman El-Ahwany , Nagwan M. Salama , Hoda Abu-Taleb , Tarek Aboushousha , Mahmoud Balata , Dina Mostafa Mohammed","doi":"10.1016/j.lfs.2025.123921","DOIUrl":null,"url":null,"abstract":"<div><div>Diabetic nephropathy is the predominant etiology of advanced renal disease, and its prevalence has increased significantly over recent years. The primary management strategy focuses on controlling identified risk factors; however, this approach only slows disease progression and cannot halt or reverse it. Therefore, novel therapeutic strategies are urgently needed. Forty-eight male rats were used in this study. After the experiment, kidney and pancreas tissues were removed and maintained for histopathological examination and gene expression analysis, and blood samples were taken to evaluate biochemical markers. Nutritional parameters were also evaluated. The diabetic nephropathy + PRP group and the diabetic nephropathy + L-GFs group showed reduced blood glucose levels and renin expression, alongside increased PDX1 and Neurog3 expression compared to the diabetic nephropathy group. Additionally, these groups exhibited decreased cytokine and malondialdehyde (MDA) levels, with significant improvements in superoxide dismutase (SOD) activity, hepatic function, and renal function biomarkers. Histopathological analysis of the kidneys and pancreas from both treatment groups showed significant improvements compared to the diabetic nephropathy group. In conclusion, administering platelet-rich plasma (PRP) and lyophilized growth factors (L-GFs) represents an innovative and promising treatment strategy for diabetic nephropathy and accompanying consequences.</div></div>","PeriodicalId":18122,"journal":{"name":"Life sciences","volume":"379 ","pages":"Article 123921"},"PeriodicalIF":5.1000,"publicationDate":"2025-08-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Life sciences","FirstCategoryId":"3","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S0024320525005569","RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"MEDICINE, RESEARCH & EXPERIMENTAL","Score":null,"Total":0}

引用次数: 0

Abstract

Diabetic nephropathy is the predominant etiology of advanced renal disease, and its prevalence has increased significantly over recent years. The primary management strategy focuses on controlling identified risk factors; however, this approach only slows disease progression and cannot halt or reverse it. Therefore, novel therapeutic strategies are urgently needed. Forty-eight male rats were used in this study. After the experiment, kidney and pancreas tissues were removed and maintained for histopathological examination and gene expression analysis, and blood samples were taken to evaluate biochemical markers. Nutritional parameters were also evaluated. The diabetic nephropathy + PRP group and the diabetic nephropathy + L-GFs group showed reduced blood glucose levels and renin expression, alongside increased PDX1 and Neurog3 expression compared to the diabetic nephropathy group. Additionally, these groups exhibited decreased cytokine and malondialdehyde (MDA) levels, with significant improvements in superoxide dismutase (SOD) activity, hepatic function, and renal function biomarkers. Histopathological analysis of the kidneys and pancreas from both treatment groups showed significant improvements compared to the diabetic nephropathy group. In conclusion, administering platelet-rich plasma (PRP) and lyophilized growth factors (L-GFs) represents an innovative and promising treatment strategy for diabetic nephropathy and accompanying consequences.

查看原文本刊更多论文

纯化血小板源性生长因子对stz诱导的糖尿病肾病和胰腺损伤中PDX1、Neurog3和肾素表达的创新治疗模式

糖尿病肾病是晚期肾脏疾病的主要病因，近年来其患病率显著增加。主要管理战略侧重于控制已确定的风险因素；然而，这种方法只能减缓疾病进展，不能停止或逆转疾病进展。因此，迫切需要新的治疗策略。本研究选用雄性大鼠48只。实验结束后，取肾、胰腺组织保存进行组织病理学检查和基因表达分析，取血进行生化指标评价。营养参数也进行了评估。与糖尿病肾病组相比，糖尿病肾病+ PRP组和糖尿病肾病+ L-GFs组血糖水平和肾素表达降低，PDX1和Neurog3表达升高。此外，这些组表现出细胞因子和丙二醛（MDA）水平降低，超氧化物歧化酶（SOD）活性、肝功能和肾功能生物标志物显著改善。与糖尿病肾病组相比，两个治疗组的肾脏和胰腺的组织病理学分析显示有显著改善。总之，给予富血小板血浆（PRP）和冻干生长因子（L-GFs）是治疗糖尿病肾病及其伴随后果的一种创新和有前途的治疗策略。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

求助全文

约1分钟内获得全文求助全文

来源期刊

Life sciences 医学-药学

CiteScore

12.20

自引率

1.60%

发文量

841

审稿时长

6 months

期刊介绍： Life Sciences is an international journal publishing articles that emphasize the molecular, cellular, and functional basis of therapy. The journal emphasizes the understanding of mechanism that is relevant to all aspects of human disease and translation to patients. All articles are rigorously reviewed. The Journal favors publication of full-length papers where modern scientific technologies are used to explain molecular, cellular and physiological mechanisms. Articles that merely report observations are rarely accepted. Recommendations from the Declaration of Helsinki or NIH guidelines for care and use of laboratory animals must be adhered to. Articles should be written at a level accessible to readers who are non-specialists in the topic of the article themselves, but who are interested in the research. The Journal welcomes reviews on topics of wide interest to investigators in the life sciences. We particularly encourage submission of brief, focused reviews containing high-quality artwork and require the use of mechanistic summary diagrams.