Genotypic diversity and growth responses to temperature and salinity variations of Coolia malayensis (Gonyaulacales, Dinophyceae) in Chinese coastal waters

IF 4.5 1区生物学 Q1 MARINE & FRESHWATER BIOLOGY

Harmful Algae Pub Date : 2025-08-14 DOI:10.1016/j.hal.2025.102945

Ruoyu Guo , Xu-Dong Lian , Ruifang Wang , Yue Jiang , Sungmo Kang , Chenjie Zhu , Xiao Ma , Shiwen Zhou , Leo Lai Chan , Xinfeng Dai , Pengbin Wang , Zhun Li

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引用次数: 0

Abstract

Coolia malayensis is a benthic dinoflagellate widely distributed in tropical to subtropical coastal waters, with some strains exhibiting cytotoxicity, indicating potential ecological and toxicological roles. This study investigated the genetic diversity and adaptability of C. malayensis strains across diverse environmental conditions in Chinese coastal waters. Three distinct genotypes, Genotype 1 (strain DF553), Genotype 2 (DF630), and Genotype 3 comprising strains DF307, DF316, and DF364, were confirmed by the large subunit (LSU) ribosomal DNA phylogenetic analysis, with a protein profiling by the matrix-assisted laser desorption/ionization-time of flight (MALDI-TOF) mass spectrometry providing supporting evidence for the phenotypic differentiations. The results of growth experiments conducted under varying temperature (15°C to 30°C) and salinity (15 to 35) conditions demonstrated genotype-specific environmental preferences: strain DF553 exhibited an optimal growth at 25°C and 30 salinity, strain DF630 at 30°C and 30, while strains DF307, DF316, and DF364 displayed a wider range of adaptive capacities. These results emphasized the ecological flexibility and adaptive potential of C. malayensis, indicating its ability to thrive in diverse marine environments. The study underscores the importance of genotypic variation in predicting species responses to environmental fluctuations, offering valuable insights for marine ecosystem management and mitigation of harmful algal blooms.

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中国沿海海域马来Coolia malayensis （Gonyaulacales, Dinophyceae）基因型多样性及其对温度和盐度变化的生长响应

马来西亚Coolia malayensis是一种广泛分布于热带至亚热带沿海水域的底栖鞭毛藻，其中一些菌株表现出细胞毒性，表明潜在的生态和毒理学作用。本研究研究了中国沿海不同环境条件下马来酸盐（C. malayensis）菌株的遗传多样性和适应性。基因1型（DF553）、基因2型（DF630）和基因3型（DF307、DF316和DF364）由LSU核糖体DNA系统发育分析证实，基质辅助激光脱附/电离飞行时间（MALDI-TOF）质谱的蛋白质谱图为表型分化提供了支持证据。在不同温度（15°C ~ 30°C）和盐度（15 ~ 35）条件下进行的生长实验结果显示了基因型特异性的环境偏好：菌株DF553在25°C和30盐度下表现出最佳生长，菌株DF630在30°C和30盐度下表现出最佳生长，而菌株DF307、DF316和DF364表现出更广泛的适应能力。这些结果强调了马来刺槐的生态灵活性和适应潜力，表明其能够在不同的海洋环境中茁壮成长。该研究强调了基因型变异在预测物种对环境波动的反应方面的重要性，为海洋生态系统管理和减轻有害藻华提供了有价值的见解。

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来源期刊

Harmful Algae 生物-海洋与淡水生物学

CiteScore

12.50

自引率

15.20%

发文量

122

审稿时长

7.5 months

期刊介绍： This journal provides a forum to promote knowledge of harmful microalgae and macroalgae, including cyanobacteria, as well as monitoring, management and control of these organisms.