Dengke Yang , Weipu Mao , Lei Jiang , Bingyan Liu , Jiang Geng
{"title":"CALD1-derived circ-0003746 targeting miR-526b promotes EMT-mediated bladder cancer progression","authors":"Dengke Yang , Weipu Mao , Lei Jiang , Bingyan Liu , Jiang Geng","doi":"10.1016/j.yexcr.2025.114710","DOIUrl":null,"url":null,"abstract":"<div><div>CALD1 is critical to bladder cancer (BCa) development. Circ-0003746 is a circRNA derived from two exons of CALD1. However, the functional role of circ-0003746 in BCa remains inadequately explored. This study investigated its involvement in BCa. Circ-0003746 was found to be overexpressed in BCa tissues and cell lines. Silencing circ-0003746 suppressed both proliferation and migration of BCa cells <em>in vitro</em> and <em>in vivo</em>. miR-526b was identified as a potential target of circ-0003746. Luciferase reporter assays confirmed the interaction between circ-0003746 and miR-526b. Mechanistically, circ-0003746 promotes epithelial-mesenchymal transition (EMT) by sequestering miR-526b, thereby advancing BCa progression. These findings highlight the role of circ-0003746 in regulating the miR-526b/EMT axis, positioning it as a potential biomarker for BCa.</div></div>","PeriodicalId":12227,"journal":{"name":"Experimental cell research","volume":"451 2","pages":"Article 114710"},"PeriodicalIF":3.5000,"publicationDate":"2025-08-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Experimental cell research","FirstCategoryId":"3","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S0014482725003106","RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q3","JCRName":"CELL BIOLOGY","Score":null,"Total":0}
引用次数: 0
Abstract
CALD1 is critical to bladder cancer (BCa) development. Circ-0003746 is a circRNA derived from two exons of CALD1. However, the functional role of circ-0003746 in BCa remains inadequately explored. This study investigated its involvement in BCa. Circ-0003746 was found to be overexpressed in BCa tissues and cell lines. Silencing circ-0003746 suppressed both proliferation and migration of BCa cells in vitro and in vivo. miR-526b was identified as a potential target of circ-0003746. Luciferase reporter assays confirmed the interaction between circ-0003746 and miR-526b. Mechanistically, circ-0003746 promotes epithelial-mesenchymal transition (EMT) by sequestering miR-526b, thereby advancing BCa progression. These findings highlight the role of circ-0003746 in regulating the miR-526b/EMT axis, positioning it as a potential biomarker for BCa.
期刊介绍:
Our scope includes but is not limited to areas such as: Chromosome biology; Chromatin and epigenetics; DNA repair; Gene regulation; Nuclear import-export; RNA processing; Non-coding RNAs; Organelle biology; The cytoskeleton; Intracellular trafficking; Cell-cell and cell-matrix interactions; Cell motility and migration; Cell proliferation; Cellular differentiation; Signal transduction; Programmed cell death.