Genome-wide identification and characterization of the universal stress protein (USP) gene family in the AC genome of Brassica species.

Abstract

Universal Stress Proteins (USPs) are widely distributed across various organisms and play a crucial role in survival under stress conditions. As environmental stresses become more severe, understanding the role of USPs in developing stress-resistant plants has gained increasing importance. In this study, we identified 231 USP-coding genes in the genomes of Brassica napus (BnUSP1-BnUSP115), B. rapa (BrUSP1-BrUSP54), and B. oleracea (BoUSP1-BoUSP62) using bioinformatics approaches. Phylogenetic analysis grouped these genes into six distinct clusters based on bootstrap values. Structural analysis of USP genes in these Brassica species revealed variability in intron numbers, with phase 0 introns being more prevalent than phases 1 and 2. Gene duplication analysis showed that segmental/WGD duplication events significantly contributed to the expansion of the USP gene family, with duplicated genes undergoing purifying selection. Promoter analysis identified several cis-regulatory elements related to stress and hormone responses-such as MYB, MYC, ARE, ERF, ABRE, TGA-element, and TCA-element-in the upstream regions of BnUSP, BoUSP, and BrUSP genes, suggesting their involvement in complex stress response pathways. Finally, RNA-seq data were used to examine the expression patterns of BnUSP genes across different tissues (root, stem, seed, flower, pod, and leaf) and under various abiotic stresses (cold, salinity, dehydration, and ABA). Their responses to salt stress were further validated using qRT-PCR. These analyses identified BnUSP60 and BnUSP2 as potential targets for breeding programs aimed at enhancing stress resistance in B. napus.