Multi-residue screening method of 195 drugs of abuse and metabolites in whole blood using LC-MS/MS and application to real blood samples

IF 3.1 3区医学 Q2 CHEMISTRY, ANALYTICAL

Journal of pharmaceutical and biomedical analysis Pub Date : 2025-08-08 DOI:10.1016/j.jpba.2025.117096

Sangeun Lee , Jihyun Lee , Hangji Ok , Eunbin Ryu , Wonhee Koo , Yoon Hee Lee , Jeasung Pyo , Na Young Lim , Chan Hyeok Kwon , Su Jeong Park , Kikyung Jung , Yoonsook Eom , Younghoon Chon , Min-Kyu Song , Youngmin Hong , Eunyoung Han

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引用次数: 0

Abstract

Polydrug use is a growing concern in the forensic field due to its high risk of overdose and its significant impact on physical and psychological health. Hence, the development of methods for simultaneously analyzing multiple drugs in blood has become increasingly essential recently. The aim of this study is to develop a liquid chromatography-tandem mass spectrometry (LC-MS/MS) method for simultaneous detection of 195 drugs of abuse (DOAs), including amphetamines, opiates, cathinones, phencyclidines, synthetic cannabinoids, cocaine, and metabolites, in 100 μL of blood. Blood samples were extracted using liquid-liquid extraction (LLE) with acetonitrile containing 0.1 % trifluoroacetic acid, and multiple reaction monitoring (MRM) was employed for the detection of 195 drugs and metabolites in a single run. The method was validated over a concentration range of 1–100 ng/mL for all target compounds, showing good linearity with a coefficient of determination (R²) greater than 0.99 for 193 compounds. The limits of quantification (LOQs) of target analytes ranged 0.1–10 ng/mL. The method was successfully applied to 20 real blood samples, detecting 22 drugs of abuse and their metabolites, with concentrations ranging from 0.2 to 401.4 ng/mL. This developed method is expected to be highly applicable in the forensic or clinical field for the rapid detection of polydrug use.

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LC-MS/MS全血195种滥用药物及代谢物多残留筛选方法及在真实血液样品中的应用

多种药物的使用是法医领域日益关注的问题，因为其过量使用的高风险及其对身心健康的重大影响。因此，近年来开发同时分析血液中多种药物的方法变得越来越重要。本研究旨在建立液相色谱-串联质谱（LC-MS/MS）同时检测100 μL血液中195种滥用药物（DOAs）的方法，包括安非他明、阿片类药物、卡西酮类药物、苯环利定、合成大麻素、可卡因和代谢物。采用含0.1 %三氟乙酸的乙腈液液萃取法（LLE）提取血样，采用多反应监测法（MRM）单趟检测195种药物及代谢物。在1 ~ 100 ng/mL的浓度范围内，对193种化合物均具有良好的线性关系，测定系数（R²）大于0.99。目标分析物的定量限为0.1 ~ 10 ng/mL。该方法成功应用于20份真实血液样本，检测出22种滥用药物及其代谢物，浓度范围为0.2 ~ 401.4 ng/mL。该方法有望广泛应用于法医或临床领域，用于多种药物的快速检测。

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来源期刊

Journal of pharmaceutical and biomedical analysis 医学-分析化学

CiteScore

6.70

自引率

5.90%

发文量

588

审稿时长

37 days

期刊介绍： This journal is an international medium directed towards the needs of academic, clinical, government and industrial analysis by publishing original research reports and critical reviews on pharmaceutical and biomedical analysis. It covers the interdisciplinary aspects of analysis in the pharmaceutical, biomedical and clinical sciences, including developments in analytical methodology, instrumentation, computation and interpretation. Submissions on novel applications focusing on drug purity and stability studies, pharmacokinetics, therapeutic monitoring, metabolic profiling; drug-related aspects of analytical biochemistry and forensic toxicology; quality assurance in the pharmaceutical industry are also welcome. Studies from areas of well established and poorly selective methods, such as UV-VIS spectrophotometry (including derivative and multi-wavelength measurements), basic electroanalytical (potentiometric, polarographic and voltammetric) methods, fluorimetry, flow-injection analysis, etc. are accepted for publication in exceptional cases only, if a unique and substantial advantage over presently known systems is demonstrated. The same applies to the assay of simple drug formulations by any kind of methods and the determination of drugs in biological samples based merely on spiked samples. Drug purity/stability studies should contain information on the structure elucidation of the impurities/degradants.