MicroRNAs as emerging biomarkers for chronic wound monitoring: Analytical methods for the point-of-care detection

IF 3.1 3区医学 Q2 CHEMISTRY, ANALYTICAL

Journal of pharmaceutical and biomedical analysis Pub Date : 2025-08-11 DOI:10.1016/j.jpba.2025.117110

Diego Alvarez-Rafael , Alfredo de la Escosura-Muñiz

引用次数: 0

Abstract

Chronic wounds represent a significant clinical challenge due to their prolonged healing time and susceptibility to infection and inflammation. Recent advances in molecular diagnostics have identified microRNAs as promising biomarkers for real-time monitoring of wound status, given their regulatory roles in the three phases of the healing process: inflammation, proliferation, and tissue remodeling. This review explores the potential of microRNAs as diagnostic tools for chronic wound management, with special focus on analytical strategies suitable for point-of-care (POC) detection like paper-based lateral flow assays or electrochemical techniques. We critically assess the advantages and limitations of current technologies regarding sensitivity, specificity, integration, and usability in decentralized healthcare settings.

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microrna作为慢性伤口监测的新兴生物标志物：即时检测的分析方法

慢性伤口由于其愈合时间长，易感染和炎症，是一项重大的临床挑战。由于microrna在愈合过程的三个阶段（炎症、增殖和组织重塑）中发挥着调节作用，分子诊断的最新进展已经确定了microrna作为实时监测伤口状态的有前途的生物标志物。这篇综述探讨了microrna作为慢性伤口管理诊断工具的潜力，特别关注于适合于即时护理（POC）检测的分析策略，如基于纸张的横向流动分析或电化学技术。我们批判性地评估了当前技术的优势和局限性，包括敏感性、特异性、集成度和分散式医疗环境的可用性。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Journal of pharmaceutical and biomedical analysis 医学-分析化学

CiteScore

6.70

自引率

5.90%

发文量

588

审稿时长

37 days

期刊介绍： This journal is an international medium directed towards the needs of academic, clinical, government and industrial analysis by publishing original research reports and critical reviews on pharmaceutical and biomedical analysis. It covers the interdisciplinary aspects of analysis in the pharmaceutical, biomedical and clinical sciences, including developments in analytical methodology, instrumentation, computation and interpretation. Submissions on novel applications focusing on drug purity and stability studies, pharmacokinetics, therapeutic monitoring, metabolic profiling; drug-related aspects of analytical biochemistry and forensic toxicology; quality assurance in the pharmaceutical industry are also welcome. Studies from areas of well established and poorly selective methods, such as UV-VIS spectrophotometry (including derivative and multi-wavelength measurements), basic electroanalytical (potentiometric, polarographic and voltammetric) methods, fluorimetry, flow-injection analysis, etc. are accepted for publication in exceptional cases only, if a unique and substantial advantage over presently known systems is demonstrated. The same applies to the assay of simple drug formulations by any kind of methods and the determination of drugs in biological samples based merely on spiked samples. Drug purity/stability studies should contain information on the structure elucidation of the impurities/degradants.