Infrared phenomics with 2D-COS unveils spatial heterogeneity and chemical evolution in a microbial biofilm

IF 3.1 3区化学 Q2 CHEMISTRY, ANALYTICAL

Vibrational Spectroscopy Pub Date : 2025-08-13 DOI:10.1016/j.vibspec.2025.103846

Pan Yang , Yadi Wang , Feng Geng , Junhong Lü , Xueling Li

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引用次数: 0

Abstract

Spatiotemporal mapping of the chemical dynamics of biofilm remains a fundamental challenge in microbial ecology. Here, we developed an infrared phenomics platform that integrating synchrotron-based Fourier transform infrared (SR-FTIR) microspectroscopy with two-dimensional correlation spectroscopy (2D-COS) to resolve the spatial heterogeneity and dynamic molecular gradients in Escherichia coli biofilms. By coupling hyperspectral imaging (30 μm resolution) with multivariate trajectory analysis, we revealed sequential chemical evolution: ester accumulation (1703 cm⁻¹) was found to precede enrichment of proteins (1653 cm⁻¹) and nucleic acids (1236 cm⁻¹), which was then followed by the deposition of polysaccharide (1055 cm⁻¹). This hierarchical molecular assembly was validated using principal component analysis (PCA) and cluster-based Euclidean distance metrics, thus establishing IR-2D-COS as a novel tool for the spatiotemporal analysis of microbial biofilm dynamics.

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利用2D-COS的红外表型组学揭示了微生物生物膜的空间异质性和化学演化

生物膜化学动力学的时空映射仍然是微生物生态学的一个基本挑战。在此，我们开发了一个红外表型组学平台，该平台将基于同步加速器的傅里叶变换红外（SR-FTIR）微光谱与二维相关光谱（2D-COS）相结合，来分析大肠杆菌生物膜的空间异质性和动态分子梯度。通过耦合高光谱成像（30 μm分辨率）和多变量轨迹分析，我们揭示了连续的化学进化：酯积累（1703 cm⁻¹）发生在蛋白质（1653 cm⁻¹）和核酸（1236 cm⁻¹）富集之前，然后是多糖（1055 cm⁻¹）的沉积。利用主成分分析（PCA）和基于聚类的欧几里得距离度量验证了这种分层分子组装，从而建立了IR-2D-COS作为微生物生物膜动力学时空分析的新工具。

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来源期刊

Vibrational Spectroscopy 化学-分析化学

CiteScore

4.70

自引率

4.00%

发文量

103

审稿时长

52 days

期刊介绍： Vibrational Spectroscopy provides a vehicle for the publication of original research that focuses on vibrational spectroscopy. This covers infrared, near-infrared and Raman spectroscopies and publishes papers dealing with developments in applications, theory, techniques and instrumentation. The topics covered by the journal include: Sampling techniques, Vibrational spectroscopy coupled with separation techniques, Instrumentation (Fourier transform, conventional and laser based), Data manipulation, Spectra-structure correlation and group frequencies. The application areas covered include: Analytical chemistry, Bio-organic and bio-inorganic chemistry, Organic chemistry, Inorganic chemistry, Catalysis, Environmental science, Industrial chemistry, Materials science, Physical chemistry, Polymer science, Process control, Specialized problem solving.