Enhanced regenerative and developmental potential of embryonal and stem cell-derived platelets compared to adult platelets.

IF 10.6 1区医学 Q1 CELL BIOLOGY

Cell Reports Medicine Pub Date : 2025-08-19 Epub Date: 2025-08-11 DOI:10.1016/j.xcrm.2025.102297

Baiming Huang, Le Li, Shun Yao, Yue Feng, Runqing Zhang, Wei Liang, Yuting Wu, Pei Su, Fei Wang, Wen Zhou, Hongtao Wang, Cuicui Liu, Jiaxi Zhou

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Abstract

The molecular features and multifaceted roles of platelets have been well characterized in adult mammals, but little is known about platelets at earlier developmental stages. In this study, we conduct transcriptomic and proteomic profiling of mouse embryonic platelets and find that, compared to adult platelets, they exhibit reduced classic immune-regulatory and procoagulant features but enhanced development-supporting traits. Notably, embryonic platelets interact more robustly with various cell types, including fibroblasts, and significantly accelerate refractory wound healing. Mechanistically, embryonic platelets promote fibroblast proliferation by releasing higher levels of IGF2. We also identify a CD59(a)⁺ platelet subpopulation in adult mice and humans that mimics the function of embryonic platelets. Additionally, human induced pluripotent stem cell (iPSC)-derived platelets share similar molecular and functional properties with embryonic platelets. Our findings highlight the unique multi-omics signatures and superior regenerative potential of embryonic and human induced pluripotent stem cell (hiPSC)-derived platelets, offering promising directions for tailoring platelet-based therapies to specific clinical needs.

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与成人血小板相比，胚胎和干细胞来源的血小板的再生和发育潜力增强。

在成年哺乳动物中，血小板的分子特征和多方面作用已被很好地表征，但对早期发育阶段的血小板知之甚少。在这项研究中，我们对小鼠胚胎血小板进行了转录组学和蛋白质组学分析，发现与成年血小板相比，它们表现出经典的免疫调节和促凝功能降低，但增强了发育支持特性。值得注意的是，胚胎血小板与各种细胞类型（包括成纤维细胞）的相互作用更强，并显著加速难治性伤口愈合。从机制上讲，胚胎血小板通过释放更高水平的IGF2促进成纤维细胞增殖。我们还在成年小鼠和人类中发现了一个CD59(a)+血小板亚群，它模仿胚胎血小板的功能。此外，人类诱导多能干细胞（iPSC）衍生的血小板与胚胎血小板具有相似的分子和功能特性。我们的研究结果强调了胚胎和人类诱导多能干细胞（hiPSC）衍生的血小板独特的多组学特征和优越的再生潜力，为定制基于血小板的治疗方法提供了有希望的方向，以满足特定的临床需求。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Cell Reports Medicine Biochemistry, Genetics and Molecular Biology-Biochemistry, Genetics and Molecular Biology (all)

CiteScore

15.00

自引率

1.40%

发文量

231

审稿时长

40 days

期刊介绍： Cell Reports Medicine is an esteemed open-access journal by Cell Press that publishes groundbreaking research in translational and clinical biomedical sciences, influencing human health and medicine. Our journal ensures wide visibility and accessibility, reaching scientists and clinicians across various medical disciplines. We publish original research that spans from intriguing human biology concepts to all aspects of clinical work. We encourage submissions that introduce innovative ideas, forging new paths in clinical research and practice. We also welcome studies that provide vital information, enhancing our understanding of current standards of care in diagnosis, treatment, and prognosis. This encompasses translational studies, clinical trials (including long-term follow-ups), genomics, biomarker discovery, and technological advancements that contribute to diagnostics, treatment, and healthcare. Additionally, studies based on vertebrate model organisms are within the scope of the journal, as long as they directly relate to human health and disease.