Automatic and accurate phase aberration compensation for a digital holographic microscopy system based on one-dimensional weighted curve fitting.

Abstract

We propose an automatic and accurate phase aberration compensation method for a digital holographic microscopy system. The phase aberrations are extracted by the one-dimensional weighted curve fitting method where the pre-compensated phase is obtained by the phase segmentation method based on the median absolute deviation (MAD) criterion, and the weights of the object phase and the background phase are acquired such that the object phase can be eliminated. Our approach does not require manual selection of area with no sampling and prior optical system parameters. Numerical simulation verifies that the proposed method is more accurate than the general one-dimensional curve fitting method. Experimental measurements demonstrate the effectiveness and applicability of the proposed method in USAF1951 resolution plates and living cells including BCAF breast cancer cells and HCT116 colon cancer cells.