High variability of the BoLA-DRB3 gene in Crioula Lageana cattle.

IF 2.4 3区生物学 Q2 GENETICS & HEREDITY

Gene Pub Date : 2025-09-20 Epub Date: 2025-08-08 DOI:10.1016/j.gene.2025.149719

Mariana da Silva Casa, Guillermo Giovambattista, Gabriella Bassi da Neves, Graziela Vieira Fonteque, Ellen Lara Miguel, Gianlucca Simão Nadal Ribeiro, Zigomar da Silva, Carla Ivane Ganz Vogel, Luiz Claudio Miletti, Shin-Nosuke Takeshima, Joandes Henrique Fonteque

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引用次数: 0

Abstract

The Bovine Leukocyte Antigen (BoLA) is one of the most important immunologic and genetic components of susceptibility or resistance to disease for cattle. The BoLA-DR region contains the monomorphic locus BoLA-DRA and three BoLA-DRB loci, with the BoLA-DRB3 gene being the most expressed and polymorphic. Its high variability benefits immunological responses against several diseases. This study aims to assess the genetic diversity and variability of the BoLA-DRB3 gene in the Crioula Lageana cattle breed and compare it with other locally adapted and commercial cattle breeds. DNA samples from 208 animals were used to genotype the second exon of this gene using the PCR-SBT technique, to determine the variability of BoLA-DRB3 in this cattle breed. The raw DNA sequences were analyzed using the Assign 400ATF ver.1.0.2.41 software. Principal component analysis (PCA) evaluated the relationship between the Crioula Lageana breed and other cattle breeds. Forty-four alleles were identified in the Crioula Lageana breed, resulting in an expected heterozygosity of 0.95. Statistical analysis demonstrated that this population is in Hardy-Weinberg equilibrium. PCA revealed the proximity of this breed to other Criollo cattle breeds. Future studies are necessary to evaluate the role of the BoLA-DRB3 gene polymorphisms detected in the adaptation of the Crioula Lageana, as well as to determine the BoLA haplotypes present in this native breed. The Crioula Lageana breed, despite its small population, maintains a high variability for the BoLA-DRB3 gene, which contributes to the preservation of desirable immunological characteristics.

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拉吉纳克里乌拉牛BoLA-DRB3基因的高变异性。

牛白细胞抗原（BoLA）是牛对疾病易感性或抗性的最重要的免疫和遗传成分之一。BoLA-DR区包含单态位点BoLA-DRA和3个BoLA-DRB位点，其中BoLA-DRB3基因表达最多，多态性最多。它的高可变性有利于对几种疾病的免疫反应。本研究旨在评估Crioula Lageana牛品种的BoLA-DRB3基因的遗传多样性和变异性，并将其与其他地方适应和商业牛品种进行比较。利用208头牛的DNA样本，采用PCR-SBT技术对该基因的第二外显子进行基因分型，以确定BoLA-DRB3在该牛品种中的变异性。使用Assign 400ATF ver.1.0.2.41软件分析原始DNA序列。主成分分析（PCA）评价了克拉乌拉品种与其他牛品种之间的关系。共鉴定出44个等位基因，杂合度为0.95。统计分析表明，该种群处于Hardy-Weinberg平衡状态。PCA显示该品种与其他克里奥罗牛品种接近。未来的研究有必要评估BoLA- drb3基因多态性在拉吉亚纳Crioula Lageana适应中的作用，并确定该本土品种中存在的BoLA单倍型。Crioula Lageana品种尽管种群较少，但保持了BoLA-DRB3基因的高变异性，这有助于保持理想的免疫特性。

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来源期刊

Gene 生物-遗传学

CiteScore

6.10

自引率

2.90%

发文量

718

审稿时长

42 days

期刊介绍： Gene publishes papers that focus on the regulation, expression, function and evolution of genes in all biological contexts, including all prokaryotic and eukaryotic organisms, as well as viruses.