Temporal regulation of organelle biogenesis.

IF 1.6 4区生物学 Q4 BIOCHEMISTRY & MOLECULAR BIOLOGY

Physical biology Pub Date : 2025-08-08 DOI:10.1088/1478-3975/adf9af

Aniruddha Nagarajan, Smruti Dixit, Sandeep Choubey

引用次数: 0

Abstract

Organelle abundance in cells is tightly regulated in response to external stimuli, but the underlying mechanisms remain poorly understood. Time-lapse imaging of fluorescently labelled organelles enables single-cell measurements of organelle copy numbers, revealing the time evolution of their distribution across a cell population. Building on a recently proposed kinetic model of organelle biogenesis, which incorporates de novo synthesis, fission, fusion, and degradation, we explore the time-dependent dynamics of organelle abundance. While previous studies focused on steady-state properties, here we calculate the first two moments of: 1) organelle copy numbers over time, and 2) first passage times to reach a specified organelle count. We show that these two moments provide a powerful means to discriminate between different mechanisms of organelle biogenesis. Notably, the time-dependent behaviour of organelle biogenesis reveals richer dynamics compared to the steady-state scenario. Our findings shed light on how cells attain steady-state organelle abundance after cell division and environmental perturbation.

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细胞器生物发生的时间调控。

细胞中的细胞器丰度受到外界刺激的严格调控，但其潜在机制尚不清楚。荧光标记细胞器的延时成像使细胞器拷贝数的单细胞测量，揭示其分布在细胞群中的时间演变。基于最近提出的细胞器生物发生的动力学模型，包括从头合成、裂变、融合和降解，我们探索了细胞器丰度的时间依赖性动力学。虽然以前的研究集中在稳态特性上，但这里我们计算了前两个时刻：1)细胞器拷贝数随时间的变化，以及2)达到指定细胞器计数的第一次传递时间。我们表明，这两个时刻提供了一个强有力的手段来区分不同的细胞器生物发生机制。值得注意的是，与稳态情景相比，细胞器生物发生的时间依赖性行为揭示了更丰富的动力学。我们的发现揭示了细胞如何在细胞分裂和环境扰动后获得稳态细胞器丰度。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Physical biology 生物-生物物理

CiteScore

4.20

自引率

0.00%

发文量

审稿时长

3 months

期刊介绍： Physical Biology publishes articles in the broad interdisciplinary field bridging biology with the physical sciences and engineering. This journal focuses on research in which quantitative approaches – experimental, theoretical and modeling – lead to new insights into biological systems at all scales of space and time, and all levels of organizational complexity. Physical Biology accepts contributions from a wide range of biological sub-fields, including topics such as: molecular biophysics, including single molecule studies, protein-protein and protein-DNA interactions subcellular structures, organelle dynamics, membranes, protein assemblies, chromosome structure intracellular processes, e.g. cytoskeleton dynamics, cellular transport, cell division systems biology, e.g. signaling, gene regulation and metabolic networks cells and their microenvironment, e.g. cell mechanics and motility, chemotaxis, extracellular matrix, biofilms cell-material interactions, e.g. biointerfaces, electrical stimulation and sensing, endocytosis cell-cell interactions, cell aggregates, organoids, tissues and organs developmental dynamics, including pattern formation and morphogenesis physical and evolutionary aspects of disease, e.g. cancer progression, amyloid formation neuronal systems, including information processing by networks, memory and learning population dynamics, ecology, and evolution collective action and emergence of collective phenomena.